Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13747
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dc.contributor.authorKhoo, Teng Kew-
dc.contributor.authorNoordin, Rahmah-
dc.contributor.authorSanthanam, Amutha-
dc.date.accessioned2012-04-03T06:28:49Z-
dc.date.available2012-04-03T06:28:49Z-
dc.date.issued2012-04-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/13747-
dc.description256-264en_US
dc.description.abstractA rapid antibody detection test is very useful for the detection of lymphatic filariasis, especially for certification and surveillance of post-mass drug administration. panLF Rapid kit is suitable for this purpose since it can detect all species of lymphatic filaria. It is based on the detection of anti-filarial IgG4 antibodies that react with recombinant B. malayi antigens, BmR1 and BmSXP. There is an increase demand for the test due to its attributes of being rapid, sensitive and specific results, as well as its field-applicability. The main aim of this paper is to obtain high recovery and purity of recombinant antigen BmSXP via a modified method of immobilized metal affinity chromatography (IMAC). The highest product yield of 11.82 mg/g dry cell weight (DCW) was obtained when IMAC was performed using the optimized protocol of 10 mM imidazole concentration in lysis buffer, 30 mM imidazole concentration in wash buffer, and 10 column volume wash buffer containing 300 mM salt concentration. This gave a 54% protein recovery improvement over the manufacturer’s protocol which recorded a product yield of only 7.68 mg/g DCW. The recovered BmSXP recombinant antigen showed good western blot reactivity, high sensitivity (31/32, 97%) and specificity (32/32, 100%) in ELISA, thus attesting to its good purity and quality.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.50(04) [April 2012]en_US
dc.subjectBmSXP Recombinant Antigenen_US
dc.subjectLymphatic Filariasisen_US
dc.subjectPanLF Rapiden_US
dc.subjectPurification Optimizationsen_US
dc.titlePurification of recombinant antigen BmSXP used in panLF Rapid kit for lymphatic filariasisen_US
dc.typeArticleen_US
Appears in Collections:IJEB Vol.50(04) [April 2012]

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