|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(1) [February 2012] >
| Title: | Purification and characterization of phytase with a wide pH adaptation from common edible mushroom Volvariella volvacea (Straw mushroom) |
| Authors: | Xu, Lijing
Zhang, Guoqing
Wang, Hexiang
Ng, Tzi Bun |
| Keywords: | Straw Mushroom
Phytase
Isolation
Volvariella Volvacea |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A novel phytase with a
molecular mass of 14 kDa
was
isolated from fresh fruiting bodies of the common edible mushroom Volvariella volvacea (Straw mushroom).
The isolation procedure involved successive chromatography on DEAE-cellulose, CM-cellulose,
Affi-gel blue gel, Q-Sepharose and Superdex-75. The enzyme was a monomeric protein and was
unadsorbed on DEAE-cellulose, CM-cellulose and Affi-gel blue gel, but was adsorbed on Q-Sepharose. The enzyme was purified
51.6-fold from the crude extract with 25.9% yield. Its N-terminal amino acid
sequence GEDNEHDTQA exhibited low homology to
the other reported phytases. The optimal pH and temperature of the purified enzyme was 5 and 45oC, respectively. The enzyme was quite stable over the pH
range of 3.0 to 9.0 with less than 30% change in its activity, suggesting that
it can be used in a very wide pH range. The enzyme exhibited broad substrate selectivity
towards
various phosphorylated compounds, but lacked antifungal activity against tested plant pathogens. |
| Page(s): | 49-54 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(1) [February 2012]
|
|
