Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13587
Title: Angiogenic response of advanced glycation end products (AGEs) involves PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub>
Authors: Devi, Manju S
Sudhakaran, Perumana R
Keywords: Advanced Glycation End Products (AGE s)
PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub>
Angiogenesis
Diabetes
VEGF
Issue Date: Feb-2012
Publisher: NISCAIR-CSIR, India
Abstract: Diabetes is associated with increased formation of advanced glycation end products (AGEs), which have been implicated in micro and macrovascular complications of diabetes. Our earlier reports showed proangiogenic effect of AGE-bovine serum albumin (BSA). In order to understand the mechanism of AGE-mediated angiogenesis, the possibility of involvement of peroxisome prolifeator activated receptor (PPAR) <sub><img src='/image/spc_char/gamma2.gif' border=0></sub>, a ligand activated transcription factor was examined. The angiogenic effect was studied in chick chorio allantoic membrane (CAM) and<b style=""> </b>by analyzing<b style=""> </b>angiogenic markers in human umbilical vein endothelial cells (HUVECs) in culture. The involvement of PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> was investigated using synthetic PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> agonist GW 1929 and antagonist GW 9662 and by RT-PCR.<b style=""> </b>In CAM assay, PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> antagonist GW 9662 reversed the AGE-induced effect on vascularity. In HUVECs in culture, GW 9662 reversed the effect of AGE-BSA and decreased the expression of CD 31, E-Selectin and VEGF. RT-PCR analysis showed that treatment with AGE-BSA caused upregulation of PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> mRNA levels. The reversal of the effect of AGE on angiogenesis by treatment with PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> antagonists and up-regulation of PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub> gene in HUVECs treated with AGE-BSA suggested the possible involvement of PPAR <sub><img src='/image/spc_char/gamma2.gif' border=0></sub>-dependent downstream pathway in mediating the angiogenic effect of AGE.
Description: 18-24
URI: http://hdl.handle.net/123456789/13587
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.49(1) [February 2012]

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