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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(02) [February 2012] >
| Title: | Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L. |
| Authors: | Gnanesh, A U
Krishna, V
Kumar, R Shashi
Venkatesh
Kumar, S R Santosh
Shashidhar, H E |
| Keywords: | Callus
Oryza Sativa
Plantlet Regeneration |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The Malnad region located in the Western Ghats of Karnataka is known for
the cultivation of indigenous rain fed land race cultivar of rice. The present
study was to investigate the callogenic and caulogenic potentialities of the
two indigenous rice cultivar namely Karimundaga and Kanadatumba using dehusked
mature embryo explants. For callus and shoot bud differentiation, the explants
were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-D (1–3
mg/L), IAA (1–2 mg/L), Kn (1–4 mg/L) and BAP (1–4 mg/L). The morphogenic potentialities
of the two rice cultivar differed in texture
of callus. In both the cultivar callogenic frequency was optimized at 1 mg/L
2,4-D concentration, it was 94% in Karimundaga and 58% in Kanadatumba.
Supplementation of IAA either alone (1–2 mg/L) or in combination with Kn or BAP
at 1 to 4 mg/L concentration of each induces shoot bud differentiation from the
calli. In the cultivar Karimundaga caulogenic frequency was highest
(10.60±2.55) at 1.0 mg/L IAA and 4.0 mg/L BAP concentration. While in the cultivar
Kanadatumba highest number of shoot buds (7.90±2.69) was differentiated at 1.0
mg/L IAA and 4.0 mg/L Kn concentration. The calli derived regenerants were successfully acclimatized in the
greenhouse and agro-morphological variations were evaluated. The growth
characteristics and yield related parameters exhibited by in vitro plants were lower than the in vivo plants. |
| Page(s): | 164-170 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(02) [February 2012]
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