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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(02) [February 2012] >
| Title: | Anti-apoptotic potential of gymnemic acid phospholipid complex pretreatment in Wistar rats with experimental cardiomyopathy |
| Authors: | Pathan, Rahila Ahmad
Bhandari, Uma
Javed, Saleem
Nag, Tapas C |
| Keywords: | Apoptosis
Cardiomyopathy
Doxorubicin
Gymnemic Acid Phospholipid Complex
Oxidative Stress |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Cardiomyocyte apoptosis in heart failure has been the topic of research in
many recent studies. In the present investigation, the potential
cardioprotective effect of gymnemic acid phospholipid complex (GPC) on
myocardial apoptosis and cardiac function was studied in doxorubicin (DOX; 30
mg/kg/ip/single dose)-induced cardiomyopathy model in rats. Doxorubicin induced cardiomyopathy was
evidenced by significant hemodynamic changes (increased systolic, diastolic,
mean arterial pressure and heart rate), decreased heart weight to body weight
ratio, increase in serum lactate dehydrogenase (LDH) and Ca2+
levels and decrease in myocardial Na+/K+ ATPase levels
along with caspase-3 activation. A marked reduction in glutathione, glutathione
peroxidase, glutathione reductase, glutathione-S-transferase, superoxide
dismutase and catalase levels along with increase in the levels of
thiobarbituric acids (TBARS) were also observed in rat myocardium. In addition,
DNA laddering observed on agarose gel electrophoresis and cardiac
histopathology study further supplemented myocardial apoptosis. Pre-treatment
with GPC significantly reduced DOX-induced cardiac toxicity, including
improvement of hemodynamic variables and heart weight to body weight ratio,
decreased serum Ca2+ level and LDH levels, myocardial caspase-3
levels, increased Na+/K+ ATPase levels and decreased myocardial TBARS levels and elevated
antioxidant enzymes as compared to pathogenic control group. Further, the
anti-apoptotic effect of GPC was verified by prevention of internucleosomal DNA
laddering on agarose gel electrophoresis and attenuation of histopathological
perturbations by doxorubicin. These observations demonstrate that GPC might
serve as a cardioprotective formulation in DOX-induced cardiomyopathy in rats. |
| Page(s): | 117-127 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(02) [February 2012]
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