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JSIR Vol.66 [2007] >
JSIR Vol.66(12) [December 2007] >
| Title: | Biostabilization and preparation of protein hydrolysates from delimed leather fleshings |
| Authors: | Bhaskar, N Sakhare, P Z Suresh, P V Gowda, Lalitha R Mahendrakar, N S |
| Keywords: | Amino acids Deliming Leather fleshings Hydrolysates Protex-6L |
| Issue Date: | Dec-2007 |
| Publisher: | CSIR |
| Abstract: | Wet leather fleshings (LFs) from sheep and goatskins had high alkaline pH (12.1) and ash (18.1%) due to liming of
fresh skins for leather production. Treatment of limed fleshings with 0.1% H2O2 (1:10 v/v) followed by 0.2 HCl (1:10 w/v)
solutions produced very little or no H2S. Delimed fleshings, mixed with 19.5% (v/w) Pediococcus acidolactici culture, 20%
(w/w) sugar and 2% (w/w) common salt, on fermentation at 30±2°C yield silage (protein 7%, fat 8% and ash 3.6%), which can
be used in animal feeds. Optimized conditions (enzyme, 1.5% v/w of Protex-6L; temp., 55°C; hydrolysis time, 180 min) for
hydrolyzing delimed LFs were determined by RSM. LFs yield white hydrolysate (yield, 3.5%; nitrogen, 12.4%; and fat, 1.5%
w/w), which can be used in aquaculture and animal feeds to supplement arginine, phenylalamine and tyrosine. |
| Page(s): | 1054-1063 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(12) [December 2007]
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