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Title: Biostabilization and preparation of protein hydrolysates from delimed leather fleshings
Authors: Bhaskar, N
Sakhare, P Z
Suresh, P V
Gowda, Lalitha R
Mahendrakar, N S
Keywords: Amino acids
Deliming
Leather fleshings
Hydrolysates
Protex-6L
Issue Date: Dec-2007
Publisher: CSIR
Abstract: Wet leather fleshings (LFs) from sheep and goatskins had high alkaline pH (12.1) and ash (18.1%) due to liming of fresh skins for leather production. Treatment of limed fleshings with 0.1% H2O2 (1:10 v/v) followed by 0.2 HCl (1:10 w/v) solutions produced very little or no H2S. Delimed fleshings, mixed with 19.5% (v/w) Pediococcus acidolactici culture, 20% (w/w) sugar and 2% (w/w) common salt, on fermentation at 30±2°C yield silage (protein 7%, fat 8% and ash 3.6%), which can be used in animal feeds. Optimized conditions (enzyme, 1.5% v/w of Protex-6L; temp., 55°C; hydrolysis time, 180 min) for hydrolyzing delimed LFs were determined by RSM. LFs yield white hydrolysate (yield, 3.5%; nitrogen, 12.4%; and fat, 1.5% w/w), which can be used in aquaculture and animal feeds to supplement arginine, phenylalamine and tyrosine.
Page(s): 1054-1063
ISSN: 0022-4456
Source:JSIR Vol.66(12) [December 2007]

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