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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Isolation and characterization of a multiple herbicide resistant strain [Av(MHR)Ar,Al,B,D] of diazotrophic cyanobacterium Anabaena variabilis |
| Authors: | Singh, Surendra
Datta, Pallavi
Tirkey, Archna |
| Keywords: | Biofertilizer
Cyanobacteria
Herbicides
Mutant |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present
study was aimed to isolate a multiple herbicide resistant strain [(MHR)Ar,Al,B,D]
of Anabaena variabilis
exhibiting resistance against four common rice-field herbicides, viz., Arozin,
Alachlor, Butachlor and 2,4-D. The multiple herbicide resistant (MHR) strain
was isolated by spontaneous mutational techniques. Of the four spontaneously occurring mutants, Arozin resistant
mutant (Ar-R) exhibited 92-98% survival in lethal concentrations of Arozin,
Alachlor, Butachlor and 2,4-D, and was designated as MHR strain, Av(MHR)Ar,Al,B,D. The MHR
strain exhibited faster growth rate, substantial increase in macromolecular
contents, photosynthesis, nitrogenase and glutamine synthetase (GS) activity
not only in graded concentration of herbicides but below (0-25 mg L-1)
and above the lethal dosages (25-80 mg L-1), and even in the
presence of combined lethal dosages of all four herbicides as compared to wild
type as well as Alachlor, Butachlor and 2,4-D resistant strains. The cross
resistance relationship in Arozin resistant (Ar-R) mutant strain of A. variabilis with lethal dosages of
Alachlor, Butachlor and 2,4-D did indicate that it was a single mutational
event and resistance to Arozin was associated with the acquisition of
resistance to Alachlor, Butachlor and 2,4-D. Evidently such improved Av(MHR)Ar,Al,B,D strain of
diazotrophic cyanobacterium A. variabilis under herbicide(s) stressed
agro-ecosystem, particularly rice-fields, would likely to serve as efficient
biofertilizer. |
| Page(s): | 77-85 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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