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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | In vitro plant regeneration from immature seeds of Murraya koenigii (L.) Spreng. |
| Authors: | Rani, Uzma
Sharma, M M
Ismail, Najma
Batra, Amla |
| Keywords: | In Vitro Propagation
Immature Seeds
Murraya Koenigii |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient and reproducible in
vitro protocol was developed for the large scale propagation of Murraya koenigii (L.) Spreng. from
immature seeds. Direct shoot proliferation was achieved from intact seedlings
on Murashige and Skoog (MS) medium supplemented with BAP (2.5 mg L-1).
The raised multiple shoots (19.85) were dwarf. Therefore, they were transferred
to shoot elongation medium comprising of MS salts fortified with GA3
(0.4 mg L-1). The elongated shoots (5.6 cm) were then rooted on ½ MS
medium supplemented with IAA (0.5 mg L-1). The plantlets were
hardened and acclimatized in plant growth chamber at 26±2°C temperature and 80% humidity under 16/8 h light and dark photoperiod by
cool white fluorescent tube light of about 3000 lux. Finally, the plantlets
were transferred to field conditions, where they survived with 80% survival
rate. |
| Page(s): | 108-110 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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