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Title: <i style="">In vitro</i> plant regeneration from immature seeds of <i style="">Murraya koenigii</i> (L.) Spreng.
Authors: Rani, Uzma
Sharma, M M
Ismail, Najma
Batra, Amla
Keywords: <i style="">In Vitro</i> Propagation
Immature Seeds
<i style="">Murraya Koenigii</i>
Issue Date: Jan-2012
Publisher: NISCAIR-CSIR, India
Abstract: An efficient and reproducible <i style="">in vitro</i> protocol was developed for the large scale propagation of <i style="">Murraya koenigii</i> (L.) Spreng. from immature seeds. Direct shoot proliferation was achieved from intact seedlings on Murashige and Skoog (MS) medium supplemented with BAP (2.5 mg L<sup>-1</sup>). The raised multiple shoots (19.85) were dwarf. Therefore, they were transferred to shoot elongation medium comprising of MS salts fortified with GA<sub>3</sub> (0.4 mg L<sup>-1</sup>). The elongated shoots (5.6 cm) were then rooted on ½ MS medium supplemented with IAA (0.5 mg L<sup>-1</sup>). The plantlets were hardened and acclimatized in plant growth chamber at 26±2°C temperature and 80% humidity under 16/8 h light and dark photoperiod by cool white fluorescent tube light of about 3000 lux. Finally, the plantlets were transferred to field conditions, where they survived with 80% survival rate.
Description: 108-110
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(1) [January 2012]

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