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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | In vitro shoot regeneration from cormel derived callus of gladiolus and bio-hardening of plantlets |
| Authors: | Pragya
Singh, S K
Misra, R L
Ranjan, J K |
| Keywords: | Bio-Hardening
Callus
Cormel Explant
Gladiolus
In Vitro Regeneration |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Callus mediated in vitro
propagation protocol for a novel genotype of gladiolus, viz., Pusa Urmil, has been developed. The MS medium containing 4.44
µM BAP+2.32 µM KIN+2.68 µM NAA induced
the maximum and earliest callogenesis, while medium devoid of NAA showed less
extent of callusing. The maximum regeneration frequency (58.15%) as well as the
maximum number of shoots per explant (29.63) were obtained on the medium
containing 8.88 µM BAP+9.29 µM
KIN+1.07 µM NAA. Presence of GA3
along with auxin and cytokinin promoted the elongation of shoots. The plantlets
had the maximum length of shoot (4.25 cm) in the medium containing 8.88 µM BAP+9.29 µM KIN+1.07 µM NAA+
0.58 µM GA3. The
regenerated shoots were separated and transferred to the medium containing ½
MS+4.92 µM IBA for rooting. The
plantlets, while shifted to glasshouse, were subjected to different arbuscular
mycorrhizal fungi (AMF) inoculation to enhance the ex vitro survival. The maximum survival (61.32%) was recorded for
the plantlets treated with
Glomus manihotis. The treatment with
other two strains of AMF, viz.,
Gigaspora gigantean and mixed strain,
was also at par with G. manihotis.
The maximum root length (3.99 cm) was recorded in the treatment with G. manihotis, while significantly lower
root length was observed in untreated plants. However, the maximum (13.32 cm)
shoot length was observed when the plantlets were treated with mixed strain of
AMF and was significantly higher compared to all other treatments (p≤0.05). |
| Page(s): | 99-104 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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