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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Isolation and characterization of caffeine degrading bacteria from coffee pulp |
| Authors: | Nayak, Sneha
Harshitha, M J
Maithili
Sampath, Charanya
Anilkumar, H S
Rao, C Vaman |
| Keywords: | Bacteria
Caffeine
Coffee Pulp
Gram Positive
Plasmid
Transformation |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Pure culture of a Gram-positive bacterium was isolated
from the coffee pulp and maintained on standard conventional nutrient agar
medium. The bacterium was identified as Brevibacterium
sp. (MTCC 10313) by the Institute of Microbial Technology (IMTECH),
Chandigarh, India. The bacterium was characterized with conventional tests and
used to study the tolerance to different concentration of caffeine in both
solid and liquid media. Brevibacterium sp.
was grown in a liquid minimal medium containing 1-8 g/L caffeine with glucose
and sucrose separately. The bacterium was able to tolerate up to 6 g/L of
caffeine in solid medium and 4 g/L in liquid medium. From the bacterium, a
plasmid of about 2500 bp mol wt was isolated. The isolated plasmid was used to
transform Escherichia coli DH5a and the transformed colonies were inoculated in
1 to 8 g/L of caffeine containing minimal media to see whether the plasmid was
involved in biodegradation of caffeine. It was observed that the plasmid
biodegraded caffeine up to 2 g/L in minimal media, whereas non-transformed
colonies could tolerate only up to 1 g/L caffein. Growth curves obtained in the
minimal media showed that transformed cells of E.coli DH5a have greater ability to tolerate and degrade
caffeine as compared to non-transformed cells. |
| Page(s): | 86-91 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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