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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Extracellular biosynthesis of silver nanoparticles by culture supernatant of Pseudomonas aeruginosa |
| Authors: | Jeevan, P
Ramya, K
Rena, A Edith |
| Keywords: | FTIR
Pseudomonas aeruginosa
SEM
Silver Nanoparticles
X-ray Diffraction |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The development of reliable, eco-friendly processes for the synthesis of
nanoscale materials is an important aspect of nanotechnology. Silver bionanoparticles
have been known to have inhibitory and bactericidal effects. In the present
study, authors report the extracellular synthesis of nanoparticles of silver by
reduction of aqueous Ag+ ions with the culture supernatant of Pseudomonas aeruginosa. It was found
that aqueous Ag+ ions in solution when exposed to P. aeruginosa get reduced, thereby
leading to the formation of silver nanoparticles. The formation of silver
nanoparticles was confirmed by the change in colour of the culture filtrate
from yellow to reddish brown after the addition of silver nitrate. The
morphology and uniformity of silver nanoparticles were investigated by UV-Vis
spectroscopy, X-ray diffraction and scanning electron microscope (SEM). The
interaction between protein and silver nanoparticles was analyzed using FTIR.
The process of reduction was extracellular, which makes it an easier method for
the synthesis of silver nanoparticles. These biosynthesized silver
nanoparticles were also evaluated for their antimicrobial activities against Escherichia coli and Vibrio cholerae. |
| Page(s): | 72-76 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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