NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >

Title: Extracellular biosynthesis of silver nanoparticles by culture supernatant of Pseudomonas aeruginosa
Authors: Jeevan, P
Ramya, K
Rena, A Edith
Keywords: FTIR
Pseudomonas aeruginosa
Silver Nanoparticles
X-ray Diffraction
Issue Date: Jan-2012
Publisher: NISCAIR-CSIR, India
Abstract: The development of reliable, eco-friendly processes for the synthesis of nanoscale materials is an important aspect of nanotechnology. Silver bionanoparticles have been known to have inhibitory and bactericidal effects. In the present study, authors report the extracellular synthesis of nanoparticles of silver by reduction of aqueous Ag+ ions with the culture supernatant of Pseudomonas aeruginosa. It was found that aqueous Ag+ ions in solution when exposed to P. aeruginosa get reduced, thereby leading to the formation of silver nanoparticles. The formation of silver nanoparticles was confirmed by the change in colour of the culture filtrate from yellow to reddish brown after the addition of silver nitrate. The morphology and uniformity of silver nanoparticles were investigated by UV-Vis spectroscopy, X-ray diffraction and scanning electron microscope (SEM). The interaction between protein and silver nanoparticles was analyzed using FTIR. The process of reduction was extracellular, which makes it an easier method for the synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were also evaluated for their antimicrobial activities against Escherichia coli and Vibrio cholerae.
Page(s): 72-76
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(1) [January 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(1) 72-76.pdf489.07 kBAdobe PDFView/Open
 Current Page Visits: 1635 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 165399 since 01-Sep-2015  Last updated on 27-Jun-2016Webmaster: