NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >

Title: An improved method of DNA isolation from polysaccharide rich leaves of Boswellia serrata Roxb.
Authors: Sharma, P
Purohit, S D
Keywords: Boswellia serrata
DNA Extraction
Issue Date: Jan-2012
Publisher: NISCAIR-CSIR, India
Abstract: A quick, simple and improved DNA extraction method from polysaccharide rich leaves of Boswellia serrata Roxb. has been developed. Different methods involving use of CTAB and SDS with or without modification were used. A CTAB based extraction method which uses diatomite to remove polysaccharides, such as, gums and resin, proved to be the best. This method allowed recovery of good quality DNA in sufficient quantity, suitable for complete digestion by restriction endonucleases and amplifiable in polymerase chain reaction as compared to other methods.
Page(s): 67-71
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(1) [January 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(1) 67-71.pdf102.16 kBAdobe PDFView/Open
 Current Page Visits: 838 
Recommend this item


National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 638591 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: