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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | An improved method of DNA isolation from polysaccharide rich leaves of Boswellia serrata Roxb. |
| Authors: | Sharma, P
Purohit, S D |
| Keywords: | Boswellia serrata
CTAB
Diatomite
DNA Extraction
PVP |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A quick, simple and improved DNA extraction
method from polysaccharide rich leaves of Boswellia serrata Roxb. has
been developed. Different methods involving use of CTAB and SDS with or without
modification were used. A CTAB based extraction method which uses diatomite to
remove polysaccharides, such as, gums and resin, proved to be the best. This
method allowed recovery of good quality DNA in sufficient quantity, suitable
for complete digestion by restriction endonucleases and amplifiable in
polymerase chain reaction as compared to other methods. |
| Page(s): | 67-71 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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