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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Characterization of genetic diversity in Jatropha curcas L. germplasm using RAPD and ISSR markers |
| Authors: | Khurana-Kaul, Varsha
Kachhwaha, Sumita
Kothari, S L |
| Keywords: | Genetic Diversity
ISSR
Jatropha curcas
Microsatellites
Polymorphism
RAPD |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Jatropha curcas L. is a rapidly emerging
biofuel crop attracting a lot of interest, triggering large investments and
rapid expansion of cultivation areas. In the present investigation, the genetic
relationships of 29 J. curcas
accessions were assessed based on randomly amplified polymorphic DNA (RAPD) and
inter simple sequence repeat (ISSR) analyses. A total of 72 polymorphic primers
(47 RAPD and 25 ISSR) were used. Amplification of genomic DNA of the 29 genotypes, using
RAPD analysis, yielded 552 fragments that could be scored, of which 334 were
polymorphic with an average of 7.1 polymorphic fragments per primer. Number of
amplified fragments varied from 2 to 23 and ranged in size from 100-3,500 bp.
The 25 polymorphic ISSR primers used in the study produced 336 bands across 29
genotypes, of which 201 were polymorphic. The number of amplified bands varied
from 7 to 20 with a size range of 100-3,500 bp. Molecular polymorphism was 60.5
and 59.8% with RAPD and ISSR markers, respectively. Mantel test between the two
Jaccard’s similarity matrices gave r=0.8623, showing good fit correlation
between RAPD and ISSR based similarities. Clustering of genotypes within groups
remained more or less similar in ISSR and combined data of RAPD and ISSR. |
| Page(s): | 54-61 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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