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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Genetic diversity among greengram [Vigna radiata (L.) Wilczek] genotypes varying in micronutrient (Fe and Zn) content using RAPD markers |
| Authors: | Taunk, Jyoti
Yadav, Neelam R
Yadav, Ram C
Kumar, Ram |
| Keywords: | Genetic Diversity
Greengram
Iron and Zinc Content
Micronutrient
Mungbean
RAPD |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Vigna radiata (L.) Wilczek, commonly known
as greengram or mungbean, is an
important food legume that has inexpensive, low flatulence protein, which makes
it indispensable in Indian vegetarian diet. There is a global effort to breed
and disseminate new bio-fortified varieties rich in iron (Fe) and zinc (Zn)
content to fight hidden hunger. RAPD polymorphism among 16 mungbean genotypes
using 33 decamer primers revealed 148 amplified bands with an average of 6.16
bands per primer, of which 142 were polymorphic and 6 were monomorphic. The
similarity coefficients among different genotypes ranged from 0.40-0.89 and the
genotypes were categorized into two major clusters. Fe content (mg/kg) varied
from 44.52 in GP-181 to 106.15 in Ganga-8, while Zn content (mg/kg) varied from
23.31 in Satya to 40.46 in
ML-776. High Fe and Zn genotypes did not cluster together; however, RAPD
markers were efficient for identification of genotypes—high Fe (85.1 mg/kg) and
high zinc (46.31 mg/kg) ML-776 and low Fe (46.31mg/kg) and low Zn (23.31 mg/kg)
Satya, falling in two different subgroups based on cluster analysis. These
genotypes can be used in linkage analysis by developing mapping population to
tag this trait. |
| Page(s): | 48-53 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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