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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(1) [January 2012] >
| Title: | Analysis of EMS induced in vitro mutants of Asteracantha longifolia (L.) Nees using RAPD markers |
| Authors: | Behera, Motilal
Panigrahi, Jogeswar
Mishra, Ramya Ranjan
Rath, Shiba Prasad |
| Keywords: | Asteracantha Longifolia
EMS
Genetic Variability
In Vitro Mutagenesis
RAPD |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Ethyl methyl sulfonate (EMS) treated leaf
explants were grown in vitro on MS
medium fortified with BA (8.88 µM) and NAA (2.69 µM). A total of 24 mutants
including 4 dwarf mutants, 7 leaf mutants and 13 flower mutants, selected in IM1
culture generation, were analyzed at morphological, phytochemical and molecular
level. Morphological analysis of mutants revealed significant variation in
plant height (18.6 to 42.3
cm), internode length (2.2 to 5.82), leaf size and
morphology, number of inflorescence (4 to 10), flower colour (white to violet)
and phytosterol content (0.033 to 0.0467 mg/g). RAPD analysis involving 30
primers generated 185 amplified products; of which, 86 (46.73%) were
polymorphic in nature. Jaccards similarity coefficient matrix between mutant
lines inter se and with control plant
ranges from 0.636 to
0.881. UPGMA based unrooted tree grouped all 24 mutant lines and control plant
into two major clusters, which were
sub-clustered into four, and the pattern well corresponded to the flower colour
as well as phytosterol content data baring two mutant lines ALM-12 and ALM-13. |
| Page(s): | 39-47 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(1) [January 2012]
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