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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.19 [2012] >
IJCT Vol.19(1) [January 2012] >
| Title: | Selective and validated spectrophotometric methods for determination of ganciclovir with PDAB and Folin’s reagents |
| Authors: | Kumar, T Anil
Gurupadayya, B M
Reddy, M B Rahul |
| Keywords: | Folin’s Reagent
Ganciclovir
Spectrophotometric Study
p-Dimethyl amino benzaldehyde |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Two simple, sensitive, selective, accurate
and precise methods (Methods A and B) have been developed for the quantitative
estimation of ganciclovir in bulk drug and its pharmaceutical formulations. In Method
A, presence of amino group in ganciclovir enables the use of condensation
reaction with p-dimethylamino benzaldehyde,
forming the greenish yellow colored complex due to the formation of Schiff’s
base. It shows absorption maximum at 401 nm and obeying Beer’s law in the
concentration range 80–200 μg mL-1. Method B is based on the formation
of reaction with 1,2-naphtha
quinine-4-sulphonic acid sodium salt (Folin’s Reagent), exhibiting absorption
maximum at 544 nm and obeying Beer’s law in the concentration range 4–14 μg mL-1.
The results of analysis for both the methods have been validated statistically
and by recovery studies. These results have been compared with those obtained
by using UV spectrophotometric methods developed in our laboratory. |
| Page(s): | 56-62 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.19(1) [January 2012]
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