Please use this identifier to cite or link to this item:
http://nopr.niscair.res.in/handle/123456789/13504
Title: | Selective and validated spectrophotometric methods for determination of ganciclovir with PDAB and Folin’s reagents |
Authors: | Kumar, T Anil Gurupadayya, B M Reddy, M B Rahul |
Keywords: | Folin’s Reagent;Ganciclovir;Spectrophotometric Study;p-Dimethyl amino benzaldehyde |
Issue Date: | Jan-2012 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | Two simple, sensitive, selective, accurate and precise methods (Methods A and B) have been developed for the quantitative estimation of ganciclovir in bulk drug and its pharmaceutical formulations. In Method A, presence of amino group in ganciclovir enables the use of condensation reaction with p-dimethylamino benzaldehyde, forming the greenish yellow colored complex due to the formation of Schiff’s base. It shows absorption maximum at 401 nm and obeying Beer’s law in the concentration range 80–200 μg mL-1. Method B is based on the formation of reaction with 1,2-naphtha quinine-4-sulphonic acid sodium salt (Folin’s Reagent), exhibiting absorption maximum at 544 nm and obeying Beer’s law in the concentration range 4–14 μg mL-1. The results of analysis for both the methods have been validated statistically and by recovery studies. These results have been compared with those obtained by using UV spectrophotometric methods developed in our laboratory. |
Page(s): | 56-62 |
URI: | http://hdl.handle.net/123456789/13504 |
ISSN: | 0975-0991 (Online); 0971-457X (Print) |
Appears in Collections: | IJCT Vol.19(1) [January 2012] |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
IJCT 19(1) 56-62.pdf | 95.21 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.