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JSIR Vol.71 [2012] >
JSIR Vol.71(02) [February 2012] >
| Title: | Optimized growth of Rhodobacter sphaeroides O.U.001 using response surface methodology (RSM) |
| Authors: | Hay, Jacqueline Xiao Wen
Wu, Ta Yeong
Teh, Chee Yang
Jahim, Jamaliah Md. |
| Keywords: | Biohydrogen
Photofermentation
Response Surface Methodology (RSM)
Rhodobacter sphaeroides O.U.001 |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents optimization of growth
parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an
economical viable biohydrogen production process by Rhodobacter sphaeroides O.U.001
using response surface methodology (RSM) followed by analysis of light and
agitation effects on bacterial growth using optimized conditions. Optimized
growth conditions on third day of fermentation, when R. sphaeroides O.U.001
was cultured aerobically under the presence of light (510 lux), were found as
follows: temperature, 30.3oC; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum
size, 6.02% (v/v). Light did not give a significant effect on overall growth of
R. sphaeroides O.U.001. Agitation greatly influenced bacterial growth as
a result of higher dissolved oxygen and dispersion of macromolecules in shake
culture. |
| Page(s): | 149-154 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(02) [February 2012]
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