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Title: Optimized growth of <i>Rhodobacter sphaeroides </i>O.U.001 using response surface methodology (RSM)
Authors: Hay, Jacqueline Xiao Wen
Wu, Ta Yeong
Teh, Chee Yang
Jahim, Jamaliah Md.
Keywords: Biohydrogen
Response Surface Methodology (RSM)
<i>Rhodobacter sphaeroides </i>O.U.001
Issue Date: Feb-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents optimization of growth parameters (temperature, pH, ammonium conc. and inoculum size) to achieve an economical viable biohydrogen production process by <i>Rhodobacter sphaeroides </i>O.U.001 using response surface methodology (RSM) followed by analysis of light and agitation effects on bacterial growth using optimized conditions. Optimized growth conditions on third day of fermentation, when <i>R. sphaeroides </i>O.U.001 was cultured aerobically under the presence of light (510 lux), were found as follows: temperature, 30.3oC; pH, 4.71; ammonium conc., 0.60 g/l; and inoculum size, 6.02% (v/v). Light did not give a significant effect on overall growth of <i>R. sphaeroides </i>O.U.001. Agitation greatly influenced bacterial growth as a result of higher dissolved oxygen and dispersion of macromolecules in shake culture.
Description: 149-154
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.71(02) [February 2012]

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