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JSIR Vol.71 [2012] >
JSIR Vol.71(02) [February 2012] >
| Title: | Development and validation of HPLC method for estimation of propranolol HCl in human plasma |
| Authors: | Srikanth, M V
Ram, B Janaki
Sunil, S A
Rao, N Sreenivasa
Murthy, K V Ramana |
| Keywords: | Accuracy
Human Plasma
HPLC
Precision
Propranolol HCl |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents a
rapid, sensitive and accurate high performance liquid chromatographic (HPLC)
method for determination of propranolol HCl in human plasma. Human plasma samples
were subjected to protein precipitation by methanol and protein free plasma
samples were directly injected into HPLC C18 column. Mobile phase consisting of
a mixture of acetonitrile: pH 4.5 phosphate buffer (35:65) was delivered at a
flow rate of 1 ml/min. A good separation of propranolol HCl and diltiazem HCl
as internal standard was
achieved with retention times of 6.6 min and 9.9 min respectively. Linearity
was obtained for propranolol (conc. range 20-280 ng/ml) and followed regression
equation, Y= 0.0162 X – 0.0515. Peak areas were reproducible as indicated by low
coefficient of variation (< 2.13%). |
| Page(s): | 120-123 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(02) [February 2012]
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