NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.71 [2012] >
JSIR Vol.71(02) [February 2012] >


Title: Development and validation of HPLC method for estimation of propranolol HCl in human plasma
Authors: Srikanth, M V
Ram, B Janaki
Sunil, S A
Rao, N Sreenivasa
Murthy, K V Ramana
Keywords: Accuracy
Human Plasma
HPLC
Precision
Propranolol HCl
Issue Date: Feb-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents a rapid, sensitive and accurate high performance liquid chromatographic (HPLC) method for determination of propranolol HCl in human plasma. Human plasma samples were subjected to protein precipitation by methanol and protein free plasma samples were directly injected into HPLC C18 column. Mobile phase consisting of a mixture of acetonitrile: pH 4.5 phosphate buffer (35:65) was delivered at a flow rate of 1 ml/min. A good separation of propranolol HCl and diltiazem HCl as internal standard was achieved with retention times of 6.6 min and 9.9 min respectively. Linearity was obtained for propranolol (conc. range 20-280 ng/ml) and followed regression equation, Y= 0.0162 X – 0.0515. Peak areas were reproducible as indicated by low coefficient of variation (< 2.13%).
Page(s): 120-123
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.71(02) [February 2012]

Files in This Item:

File Description SizeFormat
JSIR 71(2) 120-123.pdf42.75 kBAdobe PDFView/Open
 Current Page Visits: 798 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 621547 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in