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Title: In vitro and in vivo biocontrol of Vibrio harveyi using indigenous bacterium, Bacillus spp.
Authors: Janarthanam, K
George, M Rosalind
John, K Riji
Jeyaseelan, M J Prince
Keywords: Shrimp
Luminescent Bacterial Infection
Vibrio Harveyi
Issue Date: Feb-2012
Publisher: NISCAIR-CSIR, India
Abstract: The present study was undertaken to find out the potential of indigenously isolated Bacillus spp. in controlling virulent luminous Vibrio harveyi infection in shrimp in vitro and in vivo. Luminescent bacteria were isolated from shrimp farms of east coast of India. It is biochemically and genetically identified as Vibrio harveyi. Two isolates of Vibrio harveyi obtained from infected shrimps were used for pathogenicity studies in juvenile shrimps and the LD50 values for the above isolates ranged between 3.7 x 106 to 8.9 x 107 CFU/ml. Indigenously isolated and identified Bacillus spp. exhibiting antagonism were used for controlling the Vibrio infection both in vivo and in vitro. In vivo antagonistic activity was studied with three selected Bacillus isolates. The same had reduced the mortality of Penaeus monodon juveniles when challenged with V. harveyi with an RPS of 87.5 - 50%. This is without affecting the multiplication of the pathogen in water. Present study found that indigenously isolated antagonistic Bacillus sp. could be used as effective biocontrol agents against pathogenic luminescent V. harveyi.
Page(s): 83-89
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Source:IJMS Vol.41(1) [February 2012]

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