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Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.41 [2012] >
IJMS Vol.41(1) [February 2012] >
| Title: | In vitro and in vivo biocontrol of Vibrio harveyi using indigenous bacterium, Bacillus spp. |
| Authors: | Janarthanam, K
George, M Rosalind
John, K Riji
Jeyaseelan, M J Prince |
| Keywords: | Shrimp
Biocontrol
Luminescent Bacterial Infection
Vibrio Harveyi
Bacillus |
| Issue Date: | Feb-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present study was undertaken to find out the potential of indigenously
isolated Bacillus spp. in controlling virulent luminous Vibrio harveyi
infection in shrimp in vitro and in vivo. Luminescent bacteria were isolated
from shrimp farms of east coast of India. It is biochemically and
genetically identified as Vibrio harveyi. Two isolates of Vibrio harveyi obtained from infected shrimps were used for pathogenicity
studies in juvenile shrimps and the LD50 values for the above isolates
ranged between 3.7 x 106 to 8.9 x 107 CFU/ml. Indigenously isolated and identified Bacillus
spp. exhibiting antagonism were used for controlling the Vibrio
infection both in vivo and in vitro. In vivo
antagonistic activity was studied with three selected Bacillus isolates.
The same had reduced the mortality of Penaeus monodon juveniles when
challenged with
V. harveyi with an RPS of 87.5 - 50%. This is without affecting
the multiplication of the pathogen in water. Present study found that
indigenously isolated antagonistic Bacillus sp. could be used as
effective biocontrol agents against pathogenic luminescent V. harveyi. |
| Page(s): | 83-89 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1033 (Online); 0379-5136 (Print) |
| Source: | IJMS Vol.41(1) [February 2012]
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