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Title: Optimization of apple pomace based medium and fermentation conditions for pigment production by Sarcina sp.
Authors: Joshi, V K
Rana, Neerja S
Keywords: Apple pomace;Fermentation;Microbial pigments;Carotenoids;Sarcina sp.
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
IPC Code: Int. cl. (2011.01)—A61K 36/00
Abstract: The growth of Sarcina sp. (isolated from water) as a source of microbial pigment on apple pomace based medium was studied. 20 g/l of apple pomace gave maximum yield of biomass and carotenoids in the apple pomace based medium. The use of glucose (0.1%) in the apple pomace based medium gave the highest yield of biomass (8.897g/l) and carotenoids (12.87mg/100g). The effect of carbon and nitrogen sources on yield of biomass and carotenoids production by Sarcina sp. (isolated from water) a dark yellow colour producing bacteria was studied in apple pomace based medium. Among the different nitrogen sources tried, potassium nitrate (0.3%) gave the maximum production of biomass (12.30 g/l) and carotenoids (12.80mg/100g). Beyond 0.3% concentration however, there was decrease both in yield and carotenoids contents. Among different pH values (from 3.0 to 6.0), a pH of 5.5 was optimized whereas, a temperature of 35oC with an incubation period of 72 h produced the highest quantity of biomass and carotenoids.
Page(s): 421-427
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJNPR Vol.2(4) [December 2011]

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