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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(01) [January 2012] >
| Title: | Optimization of medium composition for the production of mosquitocidal toxins from Bacillus thuringiensis subsp. israelensis |
| Authors: | Poopathi, Subbiah
Archana, B |
| Keywords: | Bacillus thuringiensis subsp. israelensis
Coconut cake
Cost-effectiveness
MnCl2
Mosquito larvae
Toxicity assays |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Optimization
of chicken feather (CF) based culture medium for the production of Bacillus thuringiensis subsp. israelensis (Bti) biomass in combination with the agro industrial by-product
(coconut cake, CC) and manganese chloride (MnCl2) has been
evaluated. The biomass yield of Bti spore/crystal toxin was highest (12.06 g/L) from the test medium
(CF+CC+MnCl2) compared to the reference medium (Luria Bertani,
LB). Toxicity assay with Bti produced
from the test medium against mosquito vectors (Culex quinquefasciatus, Anopheles
stephensi and Aedes aegypti) was
also satisfactory and results were comparable with bacteria produced from LB.
The results suggest that Bti can be
produced to the maximum extent possible as a potential mosquitocidal activity
as suggested by the test medium (CF+CC+MnCl2).
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| Page(s): | 65-71 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(01) [January 2012]
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