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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(01) [January 2012] >
| Title: | Ameliorative effect of daidzein: A caveolin-1 inhibitor in vascular endothelium dysfunction induced by ovariectomy |
| Authors: | Sharma, Saurabh
Singh, Manjeet
Sharma, Pyare Lal |
| Keywords: | Caveolin
Estrogen
Vascular endothelium dysfunction
Ovariectomy |
| Issue Date: | Jan-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Estrogen deficiency was produced in female Sprague-Dawley
rats by surgical removal of both the ovaries and these animals were used 4
weeks later. Endothelium-dependent and endothelium-independent relaxations due
to acetylcholine and sodium nitroprusside were observed respectively, in
isolated rat thoracic aortic ring preparation. Extent of lipid peroxidation was
measured by estimating serum TBARS. Integrity of vascular endothelium was
assessed using hematoxylin and eosin staining. Generation of nitric oxide was
measured indirectly, by estimating serum and urinary nitrite/nitrate
concentration. Ovariectomy produced significant vascular endothelial
dysfunction, measured in terms of reduced acetylcholine-induced
endothelium-dependent vasorelaxation, serum and urinary nitrite/nitrate
concentration and impairment of integrity of vascular endothelium. Administration
of daidzein (0.2 mgkg-1day-1, sc 0.4 mgkg-1day-1,
sc and 0.8 mgkg-1day-1, sc) and Atorvastatin (30 mgkg-1day-1,
po Positive Control) for one week markedly improved vascular endothelial
dysfunction due to increase in nitric oxide bioavailability perhaps by
inhibiting caveolin-1 and activation of PI3K-AKT pathway. |
| Page(s): | 28-34 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(01) [January 2012]
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