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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.18 [2011] >
IJCT Vol.18(6) [November 2011] >
| Title: | Spectrophotometric determination of lamuvidine using acidic dye and coupling reagent |
| Authors: | Reddy, M B Rahul
Gurupadayya, B M
Kumar, T Anil |
| Keywords: | 3-methyl-2-benzothiazolinone
Acidic dye
Bromophenol blue
Lamivudine
hydrazone
Spectrophotometric study |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Two simple, extraction-free spectrophotometric
methods (Method-I and Method-II) for the quantitative estimation of lamuvidine
(LMV) in bulk drug and pharmaceutical formulations (tablets) have been
developed. First method is based on the interaction of LMV with 0.1% methanolic
solution of bromophenol blue to form a stable, red-colored, ion-pair complex
showing the peak at 595 nm. Second method is based on the oxidation followed by
coupling of 3-methyl-2-benzothiazolinone hydrazone with LMV in the presence of
ferric chloride to form green-colored chromogen exhibiting absorption maximum
at 659 nm. Both the methods follow Beer’s law in the concentration range 1-8 μg
mL-1. Linear relationships with good correlation coefficients
(0.991-0.9967) are found between absorbance and corresponding concentrations of
the drug. The reliability and performance of the proposed methods are validated
statistically, the recovery ranges from 99.4 ± 0.1% to 99.9 ± 0.3%. The results
of analysis for the two methods have been validated statistically and by
recovery studies. |
| Page(s): | 431-438 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.18(6) [November 2011]
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