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Title: Spectrophotometric determination of lamuvidine using acidic dye and coupling reagent
Authors: Reddy, M B Rahul
Gurupadayya, B M
Kumar, T Anil
Keywords: 3-methyl-2-benzothiazolinone;Acidic dye;Bromophenol blue;Lamivudine;hydrazone;Spectrophotometric study
Issue Date: Nov-2011
Publisher: NISCAIR-CSIR, India
Abstract: Two simple, extraction-free spectrophotometric methods (Method-I and Method-II) for the quantitative estimation of lamuvidine (LMV) in bulk drug and pharmaceutical formulations (tablets) have been developed. First method is based on the interaction of LMV with 0.1% methanolic solution of bromophenol blue to form a stable, red-colored, ion-pair complex showing the peak at 595 nm. Second method is based on the oxidation followed by coupling of 3-methyl-2-benzothiazolinone hydrazone with LMV in the presence of ferric chloride to form green-colored chromogen exhibiting absorption maximum at 659 nm. Both the methods follow Beer’s law in the concentration range 1-8 μg mL-1. Linear relationships with good correlation coefficients (0.991-0.9967) are found between absorbance and corresponding concentrations of the drug. The reliability and performance of the proposed methods are validated statistically, the recovery ranges from 99.4 ± 0.1% to 99.9 ± 0.3%. The results of analysis for the two methods have been validated statistically and by recovery studies.
Page(s): 431-438
ISSN: 0975-0991 (Online); 0971-457X (Print)
Appears in Collections:IJCT Vol.18(6) [November 2011]

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