NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.18 [2011] >
IJCT Vol.18(6) [November 2011] >


Title: Spectrophotometric determination of lamuvidine using acidic dye and coupling reagent
Authors: Reddy, M B Rahul
Gurupadayya, B M
Kumar, T Anil
Keywords: 3-methyl-2-benzothiazolinone
Acidic dye
Bromophenol blue
Lamivudine
hydrazone
Spectrophotometric study
Issue Date: Nov-2011
Publisher: NISCAIR-CSIR, India
Abstract: Two simple, extraction-free spectrophotometric methods (Method-I and Method-II) for the quantitative estimation of lamuvidine (LMV) in bulk drug and pharmaceutical formulations (tablets) have been developed. First method is based on the interaction of LMV with 0.1% methanolic solution of bromophenol blue to form a stable, red-colored, ion-pair complex showing the peak at 595 nm. Second method is based on the oxidation followed by coupling of 3-methyl-2-benzothiazolinone hydrazone with LMV in the presence of ferric chloride to form green-colored chromogen exhibiting absorption maximum at 659 nm. Both the methods follow Beer’s law in the concentration range 1-8 μg mL-1. Linear relationships with good correlation coefficients (0.991-0.9967) are found between absorbance and corresponding concentrations of the drug. The reliability and performance of the proposed methods are validated statistically, the recovery ranges from 99.4 ± 0.1% to 99.9 ± 0.3%. The results of analysis for the two methods have been validated statistically and by recovery studies.
Page(s): 431-438
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0991 (Online); 0971-457X (Print)
Source:IJCT Vol.18(6) [November 2011]

Files in This Item:

File Description SizeFormat
IJCT 18(6) 431-438.pdf143.13 kBAdobe PDFView/Open
 Current Page Visits: 468 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 602222 since 06-Feb-2009  Last updated on 17-Oct-2014Webmaster: nopr@niscair.res.in