Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13259
Title: <i style="mso-bidi-font-style:normal"><span style="font-size:13.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Pongamia pinnata</span></i><span style="font-size:13.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB"> seed cake: A promising and inexpensive substrate for production of protease and lipase from <i style="mso-bidi-font-style: normal">Bacillus pumilus</i> SG2 on solid-state fermentation</span>
Authors: Sangeetha, R
Geetha, A
Arulpandi, I
Keywords: Lipase
Protease
Solid state fermentation
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Pongamia pinnata</span></i><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-GB"> seed cake</span>
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;mso-bidi-font-size: 9.0pt;font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-GB">The production of a protease and a lipase from <i style="mso-bidi-font-style:normal">Bacillus pumilus</i> SG2 on solid-state fermentation using <i style="mso-bidi-font-style: normal">Pongamia pinnata</i> seed cake as substrate was studied. The seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. The initial pH, incubation time and moisture content were optimized to achieve maximal enzyme production. Maximum protease production was observed at 72 h and that of the lipase at 96 h of incubation. The production of protease (9840 U/g DM) and lipase (1974 U/g DM) were maximum at pH 7.0 and at 60% moisture content. Triton X-100 (1%) was proved to be an effective extractant for the enzymes and their optimal activity was observed at alkaline pH and at 60ºC. The molecular mass of the protease and lipase was 24 and 40 kDa, respectively. Both the enzymes were found to be stable detergent additives. The study demonstrated that inexpensive and easily available <i style="mso-bidi-font-style:normal">Pongamia</i> seed cake could be used for production of industrially important enzymes, such as protease and lipase.</span>
Description: 435-439
URI: http://hdl.handle.net/123456789/13259
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(6) [December 2011]

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