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Title: SAD phasing with in-house Cu radiation using barium as anomalous scatterer
Authors: Dhanasekaran, V
Velmurugan, D
Keywords: Lysozyme;In-house phasing;Single wavelength anomalous diffraction;Redundancy
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: Phasing of lysozyme crystals using co-crystallized barium ions was performed using single-wavelength anomalous diffraction (SAD) method using Cu radiation with in-house source of data collection. As the ion binding sites vary with respect to the pH of the buffer during crystallization, the highly isomorphic forms of lysozyme crystals grown at acidic and alkaline pH were used for the study. Intrinsic sulphur anomalous signal was also utilized with anomalous signal from lower occupancy ions for phasing. The study showed that to solve the structure by SAD technique, 2.8-fold data redundancy was sufficient when barium was used as an anomalous marker in the in-house copper X-ray radiation source for data collection. Therefore, co-crystallization of proteins with barium containing salt can be a powerful tool for structure determination using lab source.
Page(s): 399-405
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(6) [December 2011]

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