Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13255
Title: Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance
Authors: Lee, Jung Hun
Sohn, Seung Ghyu
Jung, Ha Il
An, Young Jun
Lee, Jae Jin
Kang, Lin-Woo
Lee, Sang Hee
Keywords: Antibiotic resistance
Imipenem
Crystal
OXA-23
Carbapenemase
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-US">Acinetobacter baumannii</span></i>
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:gulim;mso-bidi-font-family:="" mangal;mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-US">OXA-23, a class D carbapenemase that <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:mangal;mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US">confers widespread antibiotic resistance <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:gulim;mso-bidi-font-family:mangal;mso-ansi-language:="" en-us;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US">hydrolyzes the β-lactam rings of β-lactam antibiotics<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-US">, presenting an enormous challenge to infection control, particularly in the eradication of pathogenic bacteria such as <i style="mso-bidi-font-style: normal">Acinetobacter baumannii</i>, one of six top-priority dangerous pathogens. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing carbapenemases. In this study, OXA-23<span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:gulim;mso-bidi-font-family:mangal;mso-ansi-language:="" en-us;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US"> <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US">was purified and crystallized at 298 K and X-ray diffraction data from OXA-23<span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:gulim;mso-bidi-font-family:mangal;mso-ansi-language:="" en-us;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US"> <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-US">crystal were collected at 2.03 Å resolution using synchrotron radiation. The crystal of OXA-23 belonged to space group <i style="mso-bidi-font-style:normal">P</i>4<sub>1</sub> with unit cell parameters <i style="mso-bidi-font-style:normal">a </i>= 82.47, <i style="mso-bidi-font-style:normal">b </i>= 82.47 and <i style="mso-bidi-font-style: normal">c </i>= 172.01 Å. Analysis of the packing density showed that the asymmetric unit probably contained two molecules with a solvent content of 73.64%.</span></span></span></span></span></span></span></span>
Description: 395-398
URI: http://hdl.handle.net/123456789/13255
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(6) [December 2011]

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