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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(6) [December 2011] >
| Title: | Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance |
| Authors: | Lee, Jung Hun
Sohn, Seung Ghyu
Jung, Ha Il
An, Young Jun
Lee, Jae Jin
Kang, Lin-Woo
Lee, Sang Hee |
| Keywords: | Antibiotic resistance
Imipenem
Crystal
OXA-23
Carbapenemase
Acinetobacter baumannii |
| Issue Date: | Dec-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | OXA-23, a class D carbapenemase that confers widespread antibiotic
resistance hydrolyzes the β-lactam
rings of
β-lactam antibiotics, presenting an enormous challenge to infection control, particularly in
the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous
pathogens. Thus, the enzyme is a potential target for developing antimicrobial
agents against pathogens producing carbapenemases. In this study, OXA-23 was
purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal
were collected at 2.03 Å resolution using synchrotron radiation. The crystal of
OXA-23 belonged to space group P41
with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 Å. Analysis of the packing density showed that the
asymmetric unit probably contained two molecules with a solvent content of 73.64%. |
| Page(s): | 395-398 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(6) [December 2011]
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