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Title: Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance
Authors: Lee, Jung Hun
Sohn, Seung Ghyu
Jung, Ha Il
An, Young Jun
Lee, Jae Jin
Kang, Lin-Woo
Lee, Sang Hee
Keywords: Antibiotic resistance;Imipenem;Crystal;OXA-23;Carbapenemase;Acinetobacter baumannii
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: OXA-23, a class D carbapenemase that confers widespread antibiotic resistance hydrolyzes the β-lactam rings of β-lactam antibiotics, presenting an enormous challenge to infection control, particularly in the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous pathogens. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing carbapenemases. In this study, OXA-23 was purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal were collected at 2.03 Å resolution using synchrotron radiation. The crystal of OXA-23 belonged to space group P41 with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 Å. Analysis of the packing density showed that the asymmetric unit probably contained two molecules with a solvent content of 73.64%.
Page(s): 395-398
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(6) [December 2011]

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