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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(6) [December 2011] >
| Title: | Protective effect of ellagic acid and pumpkin seed oil against methotrexate-induced small intestine damage in rats |
| Authors: | El-Boghdady, Noha A |
| Keywords: | Methotrexate
Oxidative stress
Ellagic acid
Pumpkin seed oil
Purine catabolizing enzymes
Prostaglandin E2 |
| Issue Date: | Dec-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Gastrointestinal
toxicity is one of the most serious side effects in the methotrexate (MTX)
treatment. This study was designed to investigate whether ellagic acid (EA)
and/or pumpkin seed oil (PSO) had a protective effect on MTX-induced small intestine
damage. Forty albino rats were randomized into five groups of 8 rats each.
Group I served as a normal control group. In Group II, MTX was administered as a single dose (20
mg/kg) intraperitoneally. Groups III, IV and V were pre-treated respectively with
either PSO (40 mg/kg), EA (10 mg/kg) or 0.2% DMSO (vehicle control) orally
every day by gavage for 5 days and then they received MTX. All animals were
sacrificed 5 days after the intraperitoneal injection of MTX for
histopathological examination, estimation of serum prostaglandin E2
(PGE2) level, assay of tissue malondialdehyde (MDA), reduced
glutathione (GSH) and nitric oxide (NO) levels and myloperoxidase (MPO),
xanthine oxidase (XO) and adenosine deaminase (AD) activities. Administration
of EA and/or PSO decreased the intestinal damage, PGE2, MDA and NO
levels and MPO, XO and AD activities and increased GSH level. These results
suggest that EA and PSO protect the small intestine of rats from MTX-induced
damage through their antioxidant and anti-inflammatory effects and thus have
potential as a promising drug in the prevention of undesired side effects of
MTX. |
| Page(s): | 380-387 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(6) [December 2011]
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