Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13250
Title: <span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Functional fusion expression of sunflower multicystatin in <i>E. coli</i> and its comparison with a single domain cystatin</span>
Authors: Gholizadeh, Ashraf
Kohnehrouz, B Baghban
Keywords: Cystatin
Expression
Fusion protein
Inhibitor
Sunflower multicystatin
Phytocystatin
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Identification of the molecular structure and novel biophysiological functions of plant cystatins or phytocystatins is of great interest in the field of molecular biology. The important requirements for these are the efficient production, purification and correctly folded forms of these proteins. We report here the cloning, easy expression and characterization of a sunflower multicystatin (SMC) as a functional fusion protein in <i>E. coli</i>. For the first time, the amplified cystatin coding region was expressed as a part of maltose-binding fusion protein using pMALc2X over-expression vector in TB1 strain of <i>E. coli </i>without<i> </i>affecting the recombinant bacterial growth. In comparison to the previously prepared recombinant SMC (rSMC), a high amount (~44 mg/L of bacterial cell culture) of purified fused SMC (fSMC) was obtained using single-step purification method. fSMC strongly inhibited papain activity <i>in vitro</i> as compared to <i>Celosia</i> single-domain cystatin<i>. </i>Purified fSMC may be used for basic biochemical, pharmacological or clinical studies without the cleavage of its fusion parts.</span>
Description: 375-379
URI: http://hdl.handle.net/123456789/13250
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(6) [December 2011]

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