NISCAIR Online Periodicals Repository

Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.70 [2011] >
JSIR Vol.70(12) [December 2011] >

Title: Evaluation of PEGylation reaction and purification of monoPEGylated recombinant human granulocyte colony stimulating factor
Authors: Tiwari, Krishnanand
Kattavarapu, Krishna
Shebannavar, Sunil N
Pokalwar, Santosh.
Mishra, Maheshwari K
Chauhan, Ugam Kumari S
Keywords: Bioassay
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: In this study, incubation of methoxy polyethylene glycol propionaldehyde (mPEG-ALD) with recombinant human granulocyte colony stimulating factor (rh-GCSF) in presence of  cyanoborohydride yielded various species of polyethylene glycol (PEG) conjugated GCSF as detected by size exclusion high performance liquid chromatography (SE-HPLC). These reactions were carried out at 1:5 molar ratio of rh-GCSF to PEG at 20-25°C. At 1 h of reaction, mainly monoPEGylated GCSF (62.4%) was formed. At 2 h of reaction, multi PEGylated GCSF (conc. 9%) was detected, where monoPEGylated GCSF constituted the most (73.2%). At 3 h of reaction, monoPEGylated (76.2%) and multi PEGylated GCSF (11.3%) were formed. Further, a process involving ion exchange and gel filtration chromatography were used to obtain pure monoPEGylated GCSF. Purified monoPEGylated GCSF was comparable to standard PEGylated rh-GCSF on NFS-60 cell line, suggesting retained biological activity of monoPEGylated GCSF. Further, the procedure is warranted for purification of other monoPEGylated proteins for therapeutic purpose.
Page(s): 1049-1053
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.70(12) [December 2011]

Files in This Item:

File Description SizeFormat
JSIR 70(12) 1049-1053.pdf126.44 kBAdobe PDFView/Open
 Current Page Visits: 1922 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 170986 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: