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JSIR Vol.70(12) [December 2011] >

Title: Production and optimization of detergent compatible thermostable alkaline protease from Bacillus cereus FJ10
Authors: Jabeen, Faiza
Qazi, Javed Iqbal
Keywords: Bacillus cereus
Detergent compatible enzyme
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: This study presents production and optimization of detergent compatible thermostable alkaline protease from Bacillus cereus FJ10. Identification of isolate FJ10 that showed maximum growth when allowed to grow for 24 h at 50°C and pH 9.0 with 1% inoculum was confirmed by 16S rRNA gene sequencing. Proteases yielded maximum activity at 50°C having pH 10.0 and 20% substrate concentration. B. cereus FJ10 was partially purified by ammonium sulfate precipitation and dialysis. Both crude and partially purified enzymes destained blood spots on cotton fabrics. Alkaline and thermostable nature of bacterial proteases qualifies them for detergent formulation.
Page(s): 1042-1048
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.70(12) [December 2011]

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