Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13099
Title: <i>In situ Carica papaya</i> stem matrix and <i>Fusarium oxysporum</i> (NCBT-156) mediated bioremediation of chromium
Authors: Amatussalam, A
Abubacker, M N
Rajendran, R Babu
Keywords: Bioabsorption
Bioaccumulation
Bioremediation
Biosorption
Chromium
<i style="">Fusarium oxysporum</i>
FTIR
SDS-PAGE
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: Removal of heavy metal chromium was carried out using the fungus <i style="">Fusarium oxysporum </i>NCBT-156 strain isolated from soil of leather tanning effluent in <i style="">in situ</i> condition using potassium dichromate solution with 10 per cent Czapek-dox liquid medium. Biosorbent matrix was developed using <i>Carica papaya </i>plant dry stem to colonize the fungal strain to facilitate bioabsorption process. Bioabsorption of chromium was by metabolically mediated intracellular accumulation process. Maximum efficiency of chromium removal by biosorption upto 90 per cent was achieved at the end of 5th day of incubation (120 h of contact time) for 100 and 200 ppm concentration, upto 80 per cent for 300 and 400 ppm, and upto 65 per cent for 500 ppm to 1000 ppm concentrations with <i>p</i>H ranging from 5.8, 5.6, 5.5, 5.4 and 5.2, respectively for 100, 200, 300, 400, 500-1000 ppm concentration. SDS-PAGE protein profile showed significant difference in 34 kDa protein band after chromium absorption by the fungus. ftir spectroscopic analysis revealed that the main functional groups involved in the uptake of chromium by <i>F.</i> <i>oxysporium </i>strain were carbonyl, carboxyl, amino and hydroxyl groups.
Description: 925-931
URI: http://hdl.handle.net/123456789/13099
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(12) [December 2011]

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