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Title: Protocol for <i style="">in vitro</i> somatic embryogenesis and regeneration of rice (<i style="">Oryza sativa</i> L.)
Authors: Verma, Dipti
Joshi, Rohit
Shukla, Alok
Kumar, Pramod
Keywords: <i style="">Indica</i> rice
Mature embryo
Somatic embryogenesis
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of <i style="">indica</i> rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (for rainfed areas), Pusa Basmati-1 (aromatic basmati) and Jaya (for irrigated areas) were used. Optimum callus formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 m<i style="">M</i> in Govind, 14.0 m<i style="">M</i> in Jaya and 15.0 m<i style="">M</i> in Pusa Basmati-1. All the cultivars showed good proliferation on MS medium without hormone. In Govind, highest embryogenic response was observed in MS medium supplemented with 2, 4-D (0.4 m<i style="">M</i>) + kinetin (0.4 m<i style="">M</i>), while in Pusa Basmati-1 with 2, 4-D (0.4 m<i style="">M</i>) + kinetin (2.0 m<i style="">M</i>) and in Jaya on hormone-free MS medium. Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 m<i style="">M</i>) of BAP or hormone-free MS medium, while in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 m<i style="">M</i>). Similarly, maximum plantlets with proliferated roots were observed in Govind on hormone-free MS medium, while in Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 m<i style="">M</i>). Developed plantlets were further successfully acclimatized and grown under pot culture up to maturity. Further the yield potential of <i style="">in vitro</i> developed plants was accessed at par to the direct seeded one under pot culture. Present, protocol standardizes somatic embryogenesis and efficient regeneration of agronomically important, high yielding and diverse <i style="">indica</i> rice cultivars which can be utilized as an efficient tool for molecular studies and genetic transformation in future.
Description: 958-963
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(12) [December 2011]

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