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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(12) [December 2011] >
| Title: | Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.) |
| Authors: | Verma, Dipti
Joshi, Rohit
Shukla, Alok
Kumar, Pramod |
| Keywords: | Indica rice
Mature embryo
Somatic embryogenesis |
| Issue Date: | Dec-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Development of highly efficient and reproducible plant regeneration
system has tremendous potential to provide improved technology to assist in
genetic transformation of indica rice cultivars for their
further exploitation in selection. For the development of a highly reproducible
regeneration system through somatic embryogenesis, mature embryos of highly
popular rice cultivars i.e., Govind (for rainfed areas), Pusa Basmati-1
(aromatic basmati) and Jaya (for irrigated areas) were used. Optimum callus
formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 mM in Govind, 14.0 mM in Jaya and 15.0 mM in Pusa Basmati-1. All the cultivars showed good proliferation on
MS medium without hormone.
In Govind, highest embryogenic response was
observed in MS medium supplemented with 2, 4-D (0.4 mM) + kinetin (0.4 mM), while in Pusa Basmati-1 with 2, 4-D
(0.4 mM) + kinetin (2.0 mM) and in Jaya on hormone-free MS
medium. Excellent embryo regeneration in Govind was observed on MS medium
supplemented with low concentrations (1.1 mM) of BAP or hormone-free MS medium,
while in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium
supplemented with higher concentration of BAP (2.2 mM). Similarly, maximum plantlets with
proliferated roots were observed in Govind on hormone-free MS medium, while in
Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of
NAA (4.0 mM). Developed plantlets were further
successfully acclimatized and grown under pot culture up to maturity. Further
the yield potential of in vitro
developed plants was accessed at par to the direct seeded one under pot
culture. Present, protocol standardizes somatic embryogenesis and efficient
regeneration of agronomically important, high yielding and diverse indica rice cultivars which can be
utilized as an efficient tool for molecular studies and genetic transformation
in future. |
| Page(s): | 958-963 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(12) [December 2011]
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