NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(12) [December 2011] >

Title: Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.)
Authors: Verma, Dipti
Joshi, Rohit
Shukla, Alok
Kumar, Pramod
Keywords: Indica rice
Mature embryo
Somatic embryogenesis
Issue Date: Dec-2011
Publisher: NISCAIR-CSIR, India
Abstract: Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of indica rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (for rainfed areas), Pusa Basmati-1 (aromatic basmati) and Jaya (for irrigated areas) were used. Optimum callus formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 mM in Govind, 14.0 mM in Jaya and 15.0 mM in Pusa Basmati-1. All the cultivars showed good proliferation on MS medium without hormone. In Govind, highest embryogenic response was observed in MS medium supplemented with 2, 4-D (0.4 mM) + kinetin (0.4 mM), while in Pusa Basmati-1 with 2, 4-D (0.4 mM) + kinetin (2.0 mM) and in Jaya on hormone-free MS medium. Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 mM) of BAP or hormone-free MS medium, while in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 mM). Similarly, maximum plantlets with proliferated roots were observed in Govind on hormone-free MS medium, while in Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 mM). Developed plantlets were further successfully acclimatized and grown under pot culture up to maturity. Further the yield potential of in vitro developed plants was accessed at par to the direct seeded one under pot culture. Present, protocol standardizes somatic embryogenesis and efficient regeneration of agronomically important, high yielding and diverse indica rice cultivars which can be utilized as an efficient tool for molecular studies and genetic transformation in future.
Page(s): 958-963
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.49(12) [December 2011]

Files in This Item:

File Description SizeFormat
IJEB 49(12) 958-963.pdf378.87 kBAdobe PDFView/Open
 Current Page Visits: 762 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 168043 since 01-Sep-2015  Last updated on 29-Jun-2016Webmaster: