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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(12) [December 2011] >
| Title: | DNA extraction from crayfish exoskeleton |
| Authors: | Li, Yanhe
Wang, Weimin
Liu, Xiaolian
Luo, Wei
Zhang, Jie
Gul, Yasmeen |
| Keywords: | Crayfish exoskeleton
DNA amplification
DNA extraction |
| Issue Date: | Dec-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Crayfish
exoskeleton (CE) samples are generally less invasive and easy to be collected.
However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy
and efficient DNA extraction protocol for polymerase chain reactions. Specific
primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and
compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites
markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene
amplification, the results present usefulness of CE as an easy and convenient DNA
source for PCR-based population genetic research. |
| Page(s): | 953-957 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(12) [December 2011]
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