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|Title:||Extracellular production of L- Glutaminase by marine alkalophilic Streptomyces sp.-SBU1 isolated from Cape Comorin coast|
Rajan, R. Alexis
|Keywords:||Alkalophilic actinomycetes;Streptomyces sp.;L-glutaminase;Marine sediments|
|Abstract:||Actinomycetes were isolated from marine sediments of Cape Comorin coast (Lat.8° 21’ N and Long 77° 30’E), India for extracellular L-glutaminase production. Out of 25 isolates screened, the strain Streptomyces sp.-SBU1 showed promising L-glutaminase activity. Maximal L-glutaminase activity (18.0 U/ml) was observed in a medium supplemented with 2% NaCl (w/v) and 1% malt extract (w/v) as nitrogen source, 1% glucose (w/v) as carbon source, the initial pH of 9.0 at 30°C after 96 h of incubation was found to be optimal culture condition by the strain Streptomyces sp.-SBU1. So for only few marine actinomycetes have been explored for their L-glutaminase production. Hence the present study was initiated to optimize the media composition for L-glutaminase production.|
|ISSN:||0975-1033 (Online); 0379-5136 (Print)|
|Appears in Collections:||IJMS Vol.40(5) [October 2011]|
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