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Title: Extracellular production of L- Glutaminase by marine alkalophilic Streptomyces sp.-SBU1 isolated from Cape Comorin coast
Authors: Krishnakumar, S.
Rajan, R. Alexis
Ravikumar, S.
Keywords: Alkalophilic actinomycetes;Streptomyces sp.;L-glutaminase;Marine sediments
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: Actinomycetes were isolated from marine sediments of Cape Comorin coast (Lat.8° 21’ N and Long 77° 30’E), India for extracellular L-glutaminase production. Out of 25 isolates screened, the strain Streptomyces sp.-SBU1 showed promising L-glutaminase activity. Maximal L-glutaminase activity (18.0 U/ml) was observed in a medium supplemented with 2% NaCl (w/v) and 1% malt extract (w/v) as nitrogen source, 1% glucose (w/v) as carbon source, the initial pH of 9.0 at 30°C after 96 h of incubation was found to be optimal culture condition by the strain Streptomyces sp.-SBU1. So for only few marine actinomycetes have been explored for their L-glutaminase production. Hence the present study was initiated to optimize the media composition for L-glutaminase production.
Page(s): 717-721
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.40(5) [October 2011]

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