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JSIR Vol.66 [2007] >
JSIR Vol.66(08) [August 2007] >
| Title: | Decolorization and detoxification of textile dyes and black liquor by laccase of Cyathus bulleri |
| Authors: | Salony
Mishra, S |
| Keywords: | Black liquor
Color removal
Cyathus bulleri
Laccase
Textile dyes |
| Issue Date: | Aug-2007 |
| Publisher: | CSIR |
| Abstract: | A number of direct, vat, basic and other (Ranomill yellow and Texacid fast red A) dyes have been decolorized using
concentrated culture filtrate and purified laccase of white-rot fungus Cyathus bulleri. Decolorization (>50%) was achieved in
2-9 days using culture filtrate and this time was significantly reduced with purified laccase of high specific activity (4022 U/mg
protein). Addition of ABTS {2, 2’-azinobis (3-ethylthiazoline-6-sulfonate)}, a known mediator of laccase, resulted in further
reduction of time to a few hours with decolorization rates from 260 mg l-1 day-1 to 665 mg l-1 day-1. Spectral analysis indicated
decrease in dye concentration with no accumulation of light absorbing aromatic materials. Decolorization was accompanied by
detoxification, as shown in growth inhibition experiments with Bacillus subtilis. Addition of fungal mycelium to suitably
diluted black liquor resulted in significant (60-80%) reduction in color in 3-4 days indicating suitability of this fungus for
treatment of paper mill wastes. |
| Page(s): | 684-688 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(08) [August 2007]
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