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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(11) [November 2011] >
| Title: | Induction of apoptosis by eugenol in human breast cancer cells |
| Authors: | Vidhya, N
Devaraj, S Niranjali |
| Keywords: | Apoptosis
Breast cancer
Eugenol
MCF–7 breast cancer cell |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present study, potential anticancer effect of eugenol on inhibition
of cell proliferation and induction of apoptosis in human MCF-7 breast cancer
cells was investigated. Induction of cell death by eugenol was evaluated
following MTT assay and monitoring lactate dehydrogenase released into the
culture medium for cell viability and cytotoxicity, giemsa staining for
morphological alterations, fluorescence microscopy analysis of cells using
ethidium bromide and acridine orange and quantitation of DNA fragments for
induction of apoptosis. Effect of eugenol on intracellular redox status of the
human breast cancer cells was assessed by determining the level of glutathione
and lipid peroxidation products (TBARS). Eugenol treatment inhibited the growth
and proliferation of human MCF-7 breast cancer cells through induction of cell
death, which was dose and time dependent. Microscopic examination of eugenol
treated cells showed cell shrinkage, membrane blebbing and apoptotic body
formation. Further, eugenol treatment also depleted the level of intracellular
glutathione and increased the level of lipid peroxidation. The dose dependent
increase in the percentage of apoptotic cells and DNA fragments suggested that
apoptosis was involved in eugenol induced cell death and apoptosis might have
played a role in the chemopreventive action of eugenol. |
| Page(s): | 871-878 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(11) [November 2011]
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