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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.66 [2007] >
JSIR Vol.66(08) [August 2007] >
| Title: | Identification and phylogenetic analysis of TEM gene from soil isolates |
| Authors: | Singh, Sudheer Kumar
Patel, Anil Kumar
Ahmed, Syed Ubaid
Nampoothiri, Kesvan Madhavan
Pandey, Ashok |
| Keywords: | Genomic DNA
PCR assay
β-Lactamases
Phylogenetic analysis
Soil |
| Issue Date: | Aug-2007 |
| Publisher: | CSIR |
| Abstract: | This study presents isolation and identification of naturally occurring β-lactamase (BL) producing microorganisms from
soil samples of the Western Ghats of Kerala, India. Soil samples were plated on ampicillin supplemented nutrient agar. Genomic
DNA was amplified using a degenerate primer pair designed based on TEM gene sequence alignment. Full-length BL encoding
gene was amplified using blaTEM specific primer. PCR product was identified by sequencing and blast analysis as a blaTEM
encoding ORF. The deduced protein sequence showed 99% homology with TEM 116 gene reported from Uruguay, South
Korea and Taiwan. Phylogenic study of TEM family using DNA and protein sequences showed clustering of sequences along
with TEM 116 forming a separate cluster. The clustering of TEM 116 along with three isolates showed that these isolates might
have evolved from a common ancestor. Alignment of soil isolates along with TEM 116 showed that under natural conditions
also mutations occurred and TEM gene was present in soil isolates. |
| Page(s): | 660-666 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(08) [August 2007]
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