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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Pyrene biodegradation by free and immobilized cells of Mycobacterium frederiksbergense using a solvent encapsulated system |
| Authors: | Sarma, Saurabh Jyoti
Pakshirajan, Kannan
Saamrat, K B G |
| Keywords: | Biodegradation
Cell immobilization
Mycobacterium frederiksbergense
Polycyclic aromatic hydrocarbons
Pyrene
Solvent encapsulation |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Polycyclic
aromatic hydrocarbons (PAHs) are classified as hazardous pollutants in the
environment due to their toxic, mutagenic and recalcitrant nature. Compared to
other methods, biodegradation using microorganisms seems to be attractive
alternative for treating PAH contaminated environment. However, there are
limitations because of its low aqueous solubility and negligible
bioavailability to the degrading microbes. The present study investigated
biodegradation of pyrene, a model PAH compound, using both free and calcium
alginate immobilized cells of Mycobacterium
frederiksbergense. To overcome the bioavailability problem, organic phase (silicone oil) containing
the PAH compound was encapsulated using chitosan coated sodium alginate-polyvinyl
alcohol beads for subsequent use in the biodegradation experiments. Prior to
the biodegradation experiments, characterization of the silicone oil
encapsulated beads and the cell immobilized beads were also performed. In
pyrene biodegradation experiments using the free cells, following an initial
lag phase of 4 d, ~90% degradation was achieved at 400 and 800 mg/L initial
pyrene concentrations. On the other hand, immobilized cells in beads of ~700
 m size showed better
performance even at a very high initial concentration of 2000 mg/L. |
| Page(s): | 496-501 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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