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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | RAPD profile and rDNA sequence analysis of Talaromyces flavus and Trichoderma species |
| Authors: | Chakraborty, B N
Chakraborty, U
Sunar, K
Dey, P L |
| Keywords: | Talaromyces flavus
Trichoderma harzianum
RAPD
rDNA |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Talaromyces flavus isolated from paddy rhizosphere exhibited
phosphate solubilizing activity in vitro
and positively influenced the growth of Oryza
sativa, Cicer arietinum and Vigna radiata under green house
conditions. Besides, isolates of Trichoderma
harzianum and T. asperellum
obtained from agricultural field of Darjeeling
hills showed antifungal activities against fungal pathogens. Phylogenetic analysis of all
these isolates were carried out by sequencing the internal transcribed spacer
regions (ITS1 & ITS4) of the ribosomal DNA of T. flavus and T. harzianum
using universal primers. The sequences were aligned against ex-type strain
sequences from NCBI GenBank. Genetic relatedness among the isolates was studied
using random decamer primers. UPGMA cluster analysis showed that the similarity
coefficient ranged from 0.37 to 1 for T.
flavus and 0.68 to 1 for T. harzianum.
The open reading frame (ORF) analysis of 18S rDNA sequence of
T. harzianum revealed that the
sequence contains in total of four ORFs, encoding 159 amino acids with an
estimated molecular mass of 17.84 kDa. The sequences of ITS1 region have been
used as the reference sequence, which may be used for future study involving
the identification and taxonomy of both T.
flavus and Trichoderma isolates. |
| Page(s): | 487-495 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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