NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >


Title: RAPD profile and rDNA sequence analysis of Talaromyces flavus and Trichoderma species
Authors: Chakraborty, B N
Chakraborty, U
Sunar, K
Dey, P L
Keywords: Talaromyces flavus
Trichoderma harzianum
RAPD
rDNA
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: Talaromyces flavus isolated from paddy rhizosphere exhibited phosphate solubilizing activity in vitro and positively influenced the growth of Oryza sativa, Cicer arietinum and Vigna radiata under green house conditions. Besides, isolates of Trichoderma harzianum and T. asperellum obtained from agricultural field of Darjeeling hills showed antifungal activities against fungal pathogens. Phylogenetic analysis of all these isolates were carried out by sequencing the internal transcribed spacer regions (ITS1 & ITS4) of the ribosomal DNA of T. flavus and T. harzianum using universal primers. The sequences were aligned against ex-type strain sequences from NCBI GenBank. Genetic relatedness among the isolates was studied using random decamer primers. UPGMA cluster analysis showed that the similarity coefficient ranged from 0.37 to 1 for T. flavus and 0.68 to 1 for T. harzianum. The open reading frame (ORF) analysis of 18S rDNA sequence of T. harzianum revealed that the sequence contains in total of four ORFs, encoding 159 amino acids with an estimated molecular mass of 17.84 kDa. The sequences of ITS1 region have been used as the reference sequence, which may be used for future study involving the identification and taxonomy of both T. flavus and Trichoderma isolates.
Page(s): 487-495
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(4) [October 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(4) 487-495.pdf1.04 MBAdobe PDFView/Open
 Current Page Visits: 834 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 575625 since 06-Feb-2009  Last updated on 26-Aug-2014Webmaster: nopr@niscair.res.in