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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Cloning and expression of an intronless gene encoding endoglucanase from Aspergillus fumigatus MKU1 |
| Authors: | Meera, B
Vanitha, M C
Ramani, G
Manjula, A
Gunasekaran, P |
| Keywords: | Aspergillus fumigatus
Carboxymethyl cellulose
Cellulase
Cloning
Endoglucanase
Expression |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Endoglucanase
(carboxymethyl cellulase) production by an alkali tolerant fungus Aspergillus fumigatus MKU1 was
characterized under submerged fermentation conditions. The carboxymethyl cellulase
(CMCase) activity was optimal at pH
5.0 and 60°C. Intronless gene eng61
coding for endoglucanase from A.
fumigatus MKU1 was cloned and sequenced. The eng61 gene contains 993 bp ORF encoding a polypeptide of 330 amino
acids. In silico analysis of the
translated sequence revealed the presence of C-terminal cellulose binding
module and signal peptide cleavage site between 20th and 21st
amino acids. The eng61 was cloned
in pET30b and expressed in Escherichia
coli BL21 DE3. Thus, an intronless endoglucanase gene from an alkali
tolerant strain of A. fumigatus has
been cloned and expressed. |
| Page(s): | 480-486 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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