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Title: Cloning and expression of an intronless gene encoding endoglucanase from <i style="">Aspergillus fumigatus</i> MKU1
Authors: Meera, B
Vanitha, M C
Ramani, G
Manjula, A
Gunasekaran, P
Keywords: <i style="">Aspergillus fumigatus</i>
Carboxymethyl cellulose
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: Endoglucanase (carboxymethyl cellulase) production by an alkali tolerant fungus <i style="">Aspergillus fumigatus</i> MKU1 was characterized under submerged fermentation conditions. The carboxymethyl cellulase (CMCase) activity was optimal at <i style="">p</i>H 5.0 and 60°C. Intronless gene <i style="">eng</i>61 coding for endoglucanase from <i style="">A. fumigatus </i>MKU1 was cloned and sequenced. The <i style="">eng</i>61 gene contains 993 bp ORF encoding a polypeptide of 330 amino acids. <i style="">In silico</i> analysis of the translated sequence revealed the presence of C-terminal cellulose binding module and signal peptide cleavage site between 20<sup>th</sup> and 21<sup>st</sup> amino acids. The <i style="">eng</i>61 was cloned in pET30b and expressed in <i style="">Escherichia coli </i>BL21 DE3. Thus, an intronless endoglucanase gene from an alkali tolerant strain of <i style="">A. fumigatus </i>has been cloned and expressed.
Description: 480-486
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(4) [October 2011]

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