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|Title:||Comparative elucidation of properties of sucrase-cellobiase co-aggregate produced in media containing sucrose by Termitomyces clypeatus|
|Authors:||Banik, Samudra Prosad|
|Keywords:||Constitutive secretion;Industrial glycosidase;Regulated secretion;Sucrase-cellobiase co-aggregate;Termitomyces clypeatus|
|Abstract:||Production profiles and characterization of the sucrase-cellobiase (S-C) co-aggregates from the filamentous fungus Termitomyces clypeatus were compared in media containing 1% and 5% sucrose to understand the effect of cellular regulation over secretion and aggregation of these two industrially important glycosidases. The enzymes were secreted constitutively but in a high sucrose medium (5%), cellobiase secretion was reduced to a basal level of 0.03 U/mL. In intracellular, cell-bound and extracellular milieus, S/C ratios gradually declined in a predictable trend indicating participation of more cellobiase subunits for secretion. Sucrase was secreted via vacuoles in the fungus, following the same route as that of cellobiase and thus co-aggregates of S-C were present in the vacuolar fraction. The extracellular co-aggregates showed similar molecular sizes (>550 kDa) on zymography; however, SDS-PAGE revealed substantial difference in their subunit assemblies. Sucrase from the 5% medium showed a 2.6 times lower Km than 1% medium. These observations demonstrated the formation of a unique S-C co-assembly, optimally suited to its needs and accommodation of the constituent subunits, to be used for biotechnological applications.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.10(4) [October 2011]|
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