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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Comparative elucidation of properties of sucrase-cellobiase co-aggregate produced in media containing sucrose by Termitomyces clypeatus |
| Authors: | Banik, Samudra Prosad
Pal, Swagata
Ghorai, Shakuntala
Majumder, Rajib
Mukherjee, Soumya
Chowdhury, Sudeshna
Khowala, Suman |
| Keywords: | Constitutive secretion
Industrial glycosidase
Regulated secretion
Sucrase-cellobiase co-aggregate
Termitomyces clypeatus |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Production profiles and characterization of
the sucrase-cellobiase (S-C) co-aggregates
from the filamentous fungus Termitomyces
clypeatus were compared in media containing 1%
and 5% sucrose to understand the effect of cellular regulation over
secretion and aggregation of these two industrially important glycosidases. The
enzymes were secreted constitutively but in a high sucrose medium (5%),
cellobiase secretion was reduced to a basal
level of 0.03 U/mL. In intracellular, cell-bound
and extracellular milieus, S/C ratios gradually declined in a predictable trend
indicating participation of more cellobiase subunits for secretion. Sucrase was
secreted via vacuoles in the fungus,
following the same route as that of cellobiase and thus co-aggregates of S-C
were present in the vacuolar fraction. The extracellular
co-aggregates showed similar molecular sizes (>550
kDa) on zymography; however, SDS-PAGE revealed substantial difference in
their subunit assemblies. Sucrase from the 5% medium showed a 2.6 times lower Km
than 1% medium. These
observations demonstrated the formation of a unique S-C co-assembly, optimally
suited to its needs and accommodation of the constituent subunits, to be used
for biotechnological applications. |
| Page(s): | 468-479 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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