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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Adopting structural elements from intrinsically stable phytase— A promising strategy towards thermostable phytases |
| Authors: | Ushasree, Mrudula Vasudevan
Sumayya, Husaiba Beevi Salim
Pandey, Ashok |
| Keywords: | Histidine acid phosphatases
Intrinsic stability
 -propeller phytase |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Development of thermostable phytases through
biotechnology is a key issue in food and feed industry. Phytases ought to be
stable at elevated temperatures since it has to withstand feed and food processing
steps. Thermostable catalysts have been an area of extensive research since
long and several studies have focused on understanding the critical structural
features contributing to their stability curve. Recently, the explosion of high
resolution structure and availability of sequence information of stable
phytases have enforced the researchers to implement similar strategies
successfully to bring together these desirable traits into a single enzyme.
Nature has tailored unique stabilizing features in diverse classes of phytase,
understanding these critical elements and using a single or combination of
potential in vitro evolutionary
strategies would help phytase to reach the fitness peak in near future. Here we
review some recent studies on structural elements contributing to thermostability
in phytases of different microbial sources and to summarize the beneficial
effect of diverse criterion taken up to generate optimized phytase. |
| Page(s): | 458-467 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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