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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | In silico identification of B- and T-cell epitopes on OMPLA and LsrC from Salmonella typhi for peptide-based subunit vaccine design |
| Authors: | Prabhavathy, K
Perumal, P
SundaraBaalaji, N |
| Keywords: | Computational prediction
DRB1*0401
Epitope
Epitope design
Immunoinformatics
OMPLA
LsrC
MHC
Typhoid |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Typhoid, caused by Salmonella typhi,
has been the most common human illness. In
the present study, peptide-based subunit vaccine was developed from OMPLA and
LsrC against S. typhi. We adopted
sequence, 3-D structure and fold level in
silico analysis to predict B-cell and T-cell epitopes. The 3-D structure
was determined for OMPLA and LsrC by homology modeling and the modeled
structure was validated. One T-cell epitope from OMPLA (WQLSNSKES, 99.5%) and
one from LsrC (FIPNQTGTG, 75.31%) binds to maximum number of MHC class I and class
II alleles. They also specifically bind to HLA alleles, namely, A*0201, A*0204,
B*2705, DRB1*0101 and DRB1*0401. Molecular dynamics simulation of
DRB1*0401-epitope complex indicated that they are stable and flexible. Taken
together, the results indicate that OMPLA and LsrC are more suitable vaccine
candidates against typhoid. Similar epitopes from other human pathogens were
identified, which may provide useful information for vaccine development. |
| Page(s): | 440-451 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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