NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >

Title: In silico identification of B- and T-cell epitopes on OMPLA and LsrC from Salmonella typhi for peptide-based subunit vaccine design
Authors: Prabhavathy, K
Perumal, P
SundaraBaalaji, N
Keywords: Computational prediction
Epitope design
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: Typhoid, caused by Salmonella typhi, has been the most common human illness. In the present study, peptide-based subunit vaccine was developed from OMPLA and LsrC against S. typhi. We adopted sequence, 3-D structure and fold level in silico analysis to predict B-cell and T-cell epitopes. The 3-D structure was determined for OMPLA and LsrC by homology modeling and the modeled structure was validated. One T-cell epitope from OMPLA (WQLSNSKES, 99.5%) and one from LsrC (FIPNQTGTG, 75.31%) binds to maximum number of MHC class I and class II alleles. They also specifically bind to HLA alleles, namely, A*0201, A*0204, B*2705, DRB1*0101 and DRB1*0401. Molecular dynamics simulation of DRB1*0401-epitope complex indicated that they are stable and flexible. Taken together, the results indicate that OMPLA and LsrC are more suitable vaccine candidates against typhoid. Similar epitopes from other human pathogens were identified, which may provide useful information for vaccine development.
Page(s): 440-451
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(4) [October 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(4) 440-451.pdf823 kBAdobe PDFView/Open
 Current Page Visits: 135 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 164389 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: