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Title: In silico identification of putative drug targets in Klebsiella pneumonia MGH78578
Authors: Georrge, John J
Umrania, Valentina
Keywords: BLAST
Homologous proteins
KAAS server
Klebsiella pneumoniae
Potential drug targets
Reverse vaccinology
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: The prolonged use of antibiotics over the years has transformed many organisms into resistant to multiple drugs. Klebsiella pneumoniae MGH78578, a causative agent for respiratory and urinary tract infections, is one of the few Gram-negative bacteria, which has developed resistance to drugs. The present study was carried out to identify potential drug targets in K. pneumonia that might facilitate the discovery of novel drugs in near future. The present study has followed an in silico based approach for identification of drug targets. The comparison and analysis of proteomes of the causal organism and humans was made to screen out non-homologous proteins. Further, studies were carried out to list out essential proteins of the organism. KEGG pathway analysis was carried out to study the function of proteins. Different databases were used to find novel drug targets and various tools were used for the prediction of sub-cellular localization and membrane proteins. From the detailed analysis, 105 novel drug targets were identified, which have been involved in 24 specific pathways of K. pneumoniae MGH78578. Thirty nine proteins were predicted as outer membrane proteins, which could be used as potential vaccine candidates.
Page(s): 432-439
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(4) [October 2011]

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