NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >


Title: In silico identification of putative drug targets in Klebsiella pneumonia MGH78578
Authors: Georrge, John J
Umrania, Valentina
Keywords: BLAST
DEG
Homologous proteins
KAAS server
Klebsiella pneumoniae
Potential drug targets
Reverse vaccinology
Issue Date: Oct-2011
Publisher: NISCAIR-CSIR, India
Abstract: The prolonged use of antibiotics over the years has transformed many organisms into resistant to multiple drugs. Klebsiella pneumoniae MGH78578, a causative agent for respiratory and urinary tract infections, is one of the few Gram-negative bacteria, which has developed resistance to drugs. The present study was carried out to identify potential drug targets in K. pneumonia that might facilitate the discovery of novel drugs in near future. The present study has followed an in silico based approach for identification of drug targets. The comparison and analysis of proteomes of the causal organism and humans was made to screen out non-homologous proteins. Further, studies were carried out to list out essential proteins of the organism. KEGG pathway analysis was carried out to study the function of proteins. Different databases were used to find novel drug targets and various tools were used for the prediction of sub-cellular localization and membrane proteins. From the detailed analysis, 105 novel drug targets were identified, which have been involved in 24 specific pathways of K. pneumoniae MGH78578. Thirty nine proteins were predicted as outer membrane proteins, which could be used as potential vaccine candidates.
Page(s): 432-439
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(4) [October 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(4) 432-439.pdf185.34 kBAdobe PDFView/Open
 Current Page Visits: 768 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 620193 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in