Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/12978
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dc.contributor.authorSingh, Nivedita-
dc.contributor.authorAgrawal, Vishnu-
dc.contributor.authorPemmaraju, Suma C-
dc.contributor.authorPanwar, Richa-
dc.contributor.authorPruthi, Vikas-
dc.date.accessioned2011-10-31T10:33:15Z-
dc.date.available2011-10-31T10:33:15Z-
dc.date.issued2011-10-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/12978-
dc.description417-422en_US
dc.description.abstractIn the present investigation, biofilm formation by Candida albicans was studied on different polymeric surfaces, viz., polypropylene (PP), polystyrene (PS), polyvinylchloride (PVC), and silicone rubber (SR). Amongst these polymeric surfaces, the maximum biofilm formation was recorded to be 64.19, 50.31, and 45.09% for PS, PP, SR, respectively in comparison to PVC after 48 h using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-carboxanilide] tetrazolium reduction assay. Exopolysaccharides (EPS) production during biofilm formation, when assessed using acetone precipitation technique, was found to be 11.45, 9.41, 8.65 and 6.95 g/cm2 for PVC, PS, PP and SR, respectively. Atomic force microscopic and goniometric analysis showed maximum roughness (134 nm) and hydrophobicity (97°) for PVC. Confocal laser scanning microscopy (CLSM) studies revealed maximum biofilm thickness (117.5 µm) on PVC surface when analyzed by z-sectioning. Further, the data were confirmed by scanning electron microscopy (SEM) for biofilm growth on these biomaterials. It was observed that PVC as biomaterial is most susceptible for C. albicans biofilm formation, while material surface properties like roughness and hydrophobicity promotes C. albicans adhesion and biofilm development.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.10(4) [October 2011]en_US
dc.subjectBiofilmen_US
dc.subjectBiomaterialsen_US
dc.subjectCandida albicansen_US
dc.subjectCLSMen_US
dc.subjectXTTen_US
dc.titleImpact of infectious Candida albicans biofilm on biomaterialsen_US
dc.typeArticleen_US
Appears in Collections:IJBT Vol.10(4) [October 2011]

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