19-Jun-2013
11:49:24 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Impact of infectious Candida albicans biofilm on biomaterials |
| Authors: | Singh, Nivedita
Agrawal, Vishnu
Pemmaraju, Suma C
Panwar, Richa
Pruthi, Vikas |
| Keywords: | Biofilm
Biomaterials
Candida albicans
CLSM
XTT |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In
the present investigation, biofilm formation by Candida albicans was studied on different polymeric surfaces, viz.,
polypropylene (PP), polystyrene (PS), polyvinylchloride (PVC), and silicone
rubber (SR). Amongst these polymeric surfaces, the maximum biofilm formation
was recorded to be 64.19, 50.31, and 45.09% for PS, PP, SR, respectively in
comparison to PVC after 48 h using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-carboxanilide]
tetrazolium reduction assay. Exopolysaccharides (EPS) production during biofilm
formation, when assessed using acetone precipitation technique, was found to be
11.45, 9.41, 8.65 and 6.95  g/cm2 for PVC, PS, PP and SR,
respectively. Atomic force microscopic and goniometric analysis showed maximum
roughness (134 nm) and hydrophobicity (97°) for PVC. Confocal laser scanning microscopy (CLSM) studies revealed
maximum biofilm thickness (117.5 µm) on PVC surface when analyzed by z-sectioning.
Further, the data were confirmed by scanning electron microscopy (SEM) for
biofilm growth on these biomaterials. It was observed that PVC as biomaterial
is most susceptible for C. albicans
biofilm formation, while material surface properties like roughness and
hydrophobicity promotes C. albicans
adhesion and biofilm development. |
| Page(s): | 417-422 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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