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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(4) [October 2011] >
| Title: | Distribution of pathogenic factors in Staphylococcus aureus strains isolated from intra-mammary infections in cattle and buffaloes |
| Authors: | Singh, R S
Kumar, Ravinder
Yadav, B R |
| Keywords: | Buffalo
Cattle
Mastitis
Staphylococcus aureus
Pathogenic factors |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Staphylococcus aureus has been reported as a major
pathogen in dairy animals. The pathogenic potential of S. aureus isolates is attributed to a
combination of extracellular factors and invasive properties that are
controlled at the genomic level. In order to elucidate patterns of pathogenic
genes, 346 isolates of S. aureus were
recovered from Sahiwal, Karan Fries (cattles) and Murrah (buffalo) suffering
from intra-mammary infection. The distribution of pathogenic
genetic-determinants of isolates was evaluated using the PCR assays. All the
isolates showed variations and variable number of repeats (3-12) in Spa gene. The number of repeats was the
highest in Sahiwal isolates, followed by Karan Fries and Murrah. All the tested
isolates were positive for genetic determinants of Spa, Ebp, Eno and FnbA. The prevalence of other
pathogenic genes, namely, Fib, FnbB, Bbp and
Map was more in isolates from Sahiwal
as compared to isolates from Karan Fries and Murrah. A significant number of
isolates (82%) showed presence of capsular gene subtypes (5 & 8). The toxin
genes, namely, Sec, See, Seg
and Sei were common in all the
breeds; however, their proportion was less. Seb
and Sed were present in only cattle
originated isolates of S. aureus. Isolates of both cattle breeds
(Sahiwal and Karan Fries) showed similar patterns of pathogenic factors that could be due to
commonness or dissemination of strains among these animals. Conversely, variations in expression of pathogenicity factors in isolates of
cattle and buffaloes were observed. Overall, the isolates from Murrah were less
virulent and consisted of less proportion of pathogenic factors. However, the
comparative observation suggested that a limited number
of pathogenic factors of S. aureus
seemed to be crucial in mastitis in various animals. |
| Page(s): | 410-416 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(4) [October 2011]
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