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|Title:||Biodecolourization of azo dyes using Phanerochaete chrysosporium: Effect of culture conditions and enzyme activities|
Das, Ratul Kumar
|Keywords:||Azo dyes;Dye decolourization;Lignin peroxidase;Manganese peroxidase;Phanerochaete chrysosporium|
|Abstract:||This study presents effect of culture conditions [temp. (28-39oC) & agitation speed (0-200 rpm)] on biodecolourization of azo dyes [Direct Red - 80 (DR-80) & Mordant Blue – 9 (MB-9)] over an initial concentration range of 10-100 mg/l using white rot fungus, Phanerochaete chrysosporium in batch shake flasks. Optimum values of temperature & agitation speed, respectively, for dye decolourization were found to be: DR-80, 39oC & 200 rpm; and MB-9, 37oC & 150 rpm. Both dyes were best removed at the least initial concentration (10 mg/l). Employing Arrhenius equation, activation energies for decolourization of dyes were estimated to be: DR-80, 32.67; and MB-9, 39.12 kJ/mol. Maximum enzyme activities of LiP and MnP were found to be more with DR-80 than with MB-9. Study proved very good potential of ligninase producing fungus in decolourization of industrial wastewaters containing synthetic dyes.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.70(11) [November 2011]|
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