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JSIR Vol.70 [2011] >
JSIR Vol.70(11) [November 2011] >
| Title: | Media engineering for production of poly- -hydroxybutyrate by Bacillus firmus NII 0830 |
| Authors: | Deepthi, Sreelatha K
Binod, Parameswaran
Sindhu, Raveendran
Pandey, Ashok |
| Keywords: | Biodiesel industry
Biopolymer
Glycerol
Poly--hydroxybutyrate (PHB)
Response Surface Methodology (RSM) |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study evaluates biodiesel industry generated crude
glycerol as sole carbon source for the production of poly--hydroxybutyrate (PHB) using Bacillus firmus NII
0830. Pretreatment of crude glycerol from biodiesel industry was tried using
strong acids. Biomass yield and PHB accumulation by B.
firmus NII 0830 in submerged fermentation was compared using crude
as well as pretreated glycerol as sole carbon source.
It was observed that crude glycerol from biodiesel industry, without any
pretreatment, can be used for PHB production. The
organism when grown in mineral salt media containing crude glycerol as carbon
source gave a maximum yield of biomass (3 g/l) and PHB
(53% of cell dry wt). Response surface methodology (RSM) showed that
presence of nitrogen and phosphate in fermentation
media favored biomass production but reduced PHB production. Optimized
crude glycerol media for
maximum PHB accumulation contains ammonium sulphate (4 g/l) and K2HPO4 (0.8 g/l). |
| Page(s): | 968-975 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.70(11) [November 2011]
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