|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.70 [2011] >
JSIR Vol.70(11) [November 2011] >
| Title: | Assessing role of Cra in regulation of prpB and yahA genes of Escherichia coli in vivo using lacZ transcriptional fusions |
| Authors: | Ponmani, Thangaraj
Munavar, M Hussain |
| Keywords: | Cra
lacZ
prpB
yahA
Transcriptional fusion |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents in vivo role of Cra
(Catabolite repressor activator) protein in regulation of two selected genes (prpB
&
yahA) of Escherichia
coli. Gene prpB bears Cra binding site and yahA region binds
to Cra in vitro. Therefore, in pBR322
background, pprpB-lacZ and pyahA-lacZ transcriptional
fusions were generated and studied expression of b-galactosidase from
both fusions in cra+ and cra¯ genetic
backgrounds of E. coli. Results that both the promoters could possibly
be up-regulated by
Cra in vivo in E.
coli were discussed in relation to transcription control by accessory
transcription factor(s). |
| Page(s): | 938-944 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.70(11) [November 2011]
|
|
