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Title: Assessing role of Cra in regulation of prpB and yahA genes of Escherichia coli in vivo using lacZ transcriptional fusions
Authors: Ponmani, Thangaraj
Munavar, M Hussain
Keywords: Cra;lacZ;prpB;yahA;Transcriptional fusion
Issue Date: Nov-2011
Publisher: NISCAIR-CSIR, India
Abstract: This study presents in vivo role of Cra (Catabolite repressor activator) protein in regulation of two selected genes (prpB & yahA) of Escherichia coli. Gene prpB bears Cra binding site and yahA region binds to Cra in vitro. Therefore, in pBR322 background, pprpB-lacZ and pyahA-lacZ transcriptional fusions were generated and studied expression of b-galactosidase from both fusions in cra+ and cra¯ genetic backgrounds of E. coli. Results that both the promoters could possibly be up-regulated by Cra in vivo in E. coli were discussed in relation to transcription control by accessory transcription factor(s).
Page(s): 938-944
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.70(11) [November 2011]

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